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pyochelin synthetase [Pseudomonas aeruginosa PAO1].


LOCUS       NP_252915               1809 aa            linear   CON 24-JAN-2019
ACCESSION   NP_252915
VERSION     NP_252915.1
DBLINK      BioProject: PRJNA57945
            Assembly: GCF_000006765.1
DBSOURCE    REFSEQ: accession NC_002516.2
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa PAO1
  ORGANISM  Pseudomonas aeruginosa PAO1
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (residues 1 to 1809)
  AUTHORS   Winsor,G.L., Van Rossum,T., Lo,R., Khaira,B., Whiteside,M.D.,
            Hancock,R.E. and Brinkman,F.S.
  TITLE     Pseudomonas Genome Database: facilitating user-friendly,
            comprehensive comparisons of microbial genomes
  JOURNAL   Nucleic acids Res. 37 (DATABASE ISSUE), D483-D488 (2009)
   PUBMED   18978025
REFERENCE   2  (residues 1 to 1809)
  AUTHORS   Cirz,R.T., O'Neill,B.M., Hammond,J.A., Head,S.R. and Romesberg,F.E.
  TITLE     Defining the Pseudomonas aeruginosa SOS response and its role in
            the global response to the antibiotic ciprofloxacin
  JOURNAL   J. Bacteriol. 188 (20), 7101-7110 (2006)
   PUBMED   17015649
REFERENCE   3  (residues 1 to 1809)
  AUTHORS   Palmer,K.L., Mashburn,L.M., Singh,P.K. and Whiteley,M.
  TITLE     Cystic fibrosis sputum supports growth and cues key aspects of
            Pseudomonas aeruginosa physiology
  JOURNAL   J. Bacteriol. 187 (15), 5267-5277 (2005)
   PUBMED   16030221
REFERENCE   4  (residues 1 to 1809)
  AUTHORS   Salunkhe,P., Smart,C.H., Morgan,J.A., Panagea,S., Walshaw,M.J.,
            Hart,C.A., Geffers,R., Tummler,B. and Winstanley,C.
  TITLE     A cystic fibrosis epidemic strain of Pseudomonas aeruginosa
            displays enhanced virulence and antimicrobial resistance
  JOURNAL   J. Bacteriol. 187 (14), 4908-4920 (2005)
   PUBMED   15995206
REFERENCE   5  (residues 1 to 1809)
  AUTHORS   Filiatrault,M.J., Wagner,V.E., Bushnell,D., Haidaris,C.G.,
            Iglewski,B.H. and Passador,L.
  TITLE     Effect of anaerobiosis and nitrate on gene expression in
            Pseudomonas aeruginosa
  JOURNAL   Infect. Immun. 73 (6), 3764-3772 (2005)
   PUBMED   15908409
REFERENCE   6  (residues 1 to 1809)
  AUTHORS   Rasmussen,T.B., Bjarnsholt,T., Skindersoe,M.E., Hentzer,M.,
            Kristoffersen,P., Kote,M., Nielsen,J., Eberl,L. and Givskov,M.
  TITLE     Screening for quorum-sensing inhibitors (QSI) by use of a novel
            genetic system, the QSI selector
  JOURNAL   J. Bacteriol. 187 (5), 1799-1814 (2005)
   PUBMED   15716452
REFERENCE   7  (residues 1 to 1809)
  AUTHORS   Winsor,G.L., Lo,R., Sui,S.J., Ung,K.S., Huang,S., Cheng,D.,
            Ching,W.K., Hancock,R.E. and Brinkman,F.S.
  TITLE     Pseudomonas aeruginosa Genome Database and PseudoCap: facilitating
            community-based, continually updated, genome annotation
  JOURNAL   Nucleic acids Res. 33 (DATABASE ISSUE), D338-D343 (2005)
   PUBMED   15608211
REFERENCE   8  (residues 1 to 1809)
  AUTHORS   Wagner,V.E., Bushnell,D., Passador,L., Brooks,A.I. and
            Iglewski,B.H.
  TITLE     Microarray analysis of Pseudomonas aeruginosa quorum-sensing
            regulons: effects of growth phase and environment
  JOURNAL   J. Bacteriol. 185 (7), 2080-2095 (2003)
   PUBMED   12644477
REFERENCE   9  (residues 1 to 1809)
  AUTHORS   Schuster,M., Lostroh,C.P., Ogi,T. and Greenberg,E.P.
  TITLE     Identification, timing, and signal specificity of Pseudomonas
            aeruginosa quorum-controlled genes: a transcriptome analysis
  JOURNAL   J. Bacteriol. 185 (7), 2066-2079 (2003)
   PUBMED   12644476
REFERENCE   10 (residues 1 to 1809)
  AUTHORS   Stover,C.K., Pham,X.Q., Erwin,A.L., Mizoguchi,S.D., Warrener,P.,
            Hickey,M.J., Brinkman,F.S., Hufnagle,W.O., Kowalik,D.J., Lagrou,M.,
            Garber,R.L., Goltry,L., Tolentino,E., Westbrock-Wadman,S., Yuan,Y.,
            Brody,L.L., Coulter,S.N., Folger,K.R., Kas,A., Larbig,K., Lim,R.,
            Smith,K., Spencer,D., Wong,G.K., Wu,Z., Paulsen,I.T., Reizer,J.,
            Saier,M.H., Hancock,R.E., Lory,S. and Olson,M.V.
  TITLE     Complete genome sequence of Pseudomonas aeruginosa PA01, an
            opportunistic pathogen
  JOURNAL   Nature 406 (6799), 959-964 (2000)
   PUBMED   10984043
REFERENCE   11 (residues 1 to 1809)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2010) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   12 (residues 1 to 1809)
  AUTHORS   Winsor,G.L., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2010) Department of Molecular Biology and
            Biochemisry, Simon Fraser University, 8888 University Dr., Burnaby,
            British Columbia V5A 1S6, Canada
  REMARK    protein update by submitter
REFERENCE   13 (residues 1 to 1809)
  AUTHORS   Winsor,G.L., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2008) Department of Molecular Biology and
            Biochemisry, Simon Fraser University, 8888 University Dr., Burnaby,
            British Columbia V5A 1S6, Canada
  REMARK    protein update by submitter
REFERENCE   14 (residues 1 to 1809)
  AUTHORS   Winsor,G.L., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2006) Department of Molecular Biology and
            Biochemisry, Simon Fraser University, 8888 University Dr., Burnaby,
            British Columbia V5A 1S6, Canada
  REMARK    Sequence update by submitter
REFERENCE   15 (residues 1 to 1809)
  AUTHORS   Ung,K.S., Hancock,R.E. and Brinkman,F.S.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2003) Department of Molecular Biology and
            Biochemistry, Simon Fraser University, 8888 University Dr.,
            Burnaby, British Columbia V5A 1S6, Canada
REFERENCE   16 (residues 1 to 1809)
  AUTHORS   Stover,C.K., Pham,X.-Q.T., Erwin,A.L., Mizoguchi,S.D., Warrener,P.,
            Hickey,M.J., Brinkman,F.S.L., Hufnagle,W.O., Kowalik,D.J.,
            Lagrou,M., Garber,R.L., Goltry,L., Tolentino,E.,
            Westbrock-Wadman,S., Yuan,Y., Brody,L.L., Coulter,S.N.,
            Folger,K.R., Kas,A., Larbig,K., Lim,R.M., Smith,K.A., Spencer,D.H.,
            Wong,G.K.-S., Wu,Z., Paulsen,I.T., Reizer,J., Saier,M.H.,
            Hancock,R.E.W., Lory,S. and Olson,M.V.
  CONSRTM   Pseudomonas aeruginosa Community Annotation Project (PseudoCap)
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2000) Department of Medicine and Genetics,
            University of Washington Genome Center, University Of Washington,
            Box 352145, Seattle, WA 98195, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAG07613.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Pseudomonas aeruginosa PAO1"
                     /strain="PAO1"
                     /db_xref="taxon:208964"
     Protein         1..1809
                     /product="pyochelin synthetase"
                     /calculated_mol_wt=196954
     Region          5..53
                     /region_name="TubC_N"
                     /note="TubC N-terminal docking domain; pfam18563"
                     /db_xref="CDD:436580"
     Region          59..1360
                     /region_name="EntF"
                     /note="seryl-AMP synthase component of non-ribosomal
                     peptide synthetase [Secondary metabolites biosynthesis,
                     transport and catabolism]; COG1020"
                     /db_xref="CDD:440643"
     Region          528..1010
                     /region_name="A_NRPS_TlmIV_like"
                     /note="The adenylation domain of nonribosomal peptide
                     synthetases (NRPS), including Streptoalloteichus
                     tallysomycin biosynthesis genes; cd12114"
                     /db_xref="CDD:341279"
     Site            order(657,660..665,667..668)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341279"
     Site            order(660..661,779..780,801..806,829,892,904,907,1004)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341279"
     Region          <1351..1747
                     /region_name="PRK12467"
                     /note="peptide synthase; Provisional"
                     /db_xref="CDD:237108"
     CDS             1..1809
                     /gene="pchF"
                     /locus_tag="PA4225"
                     /coded_by="complement(NC_002516.2:4731369..4736798)"
                     /note="Product name confidence: class 1 (Function
                     experimentally demonstrated in P. aeruginosa)"
                     /transl_table=11
                     /db_xref="GeneID:880083"
CONTIG      join(WP_003148107.1:1..1809)
ORIGIN      
        1 mslgelletc rsrrielwse agrlryrapq galdaglaer lraerealle hleggpgwra
       61 epdmahqrfp ltpvqaayvl grqaafdygg nacqlyaeyd wpadtdparl eaawnamver
      121 hpmlravied nawqrvlpev pwqrltvhac agldeaafqa hlervrerld hacaaldqwp
      181 vlrpelsigr dacvlhcsvd ftlvdyaslq lllgewrrry ldpqwtaepl eatfrdyvgv
      241 eqrrrqspaw qrdrdwwlar ldalpgrpdl plrvqpdtrs trfrhfharl deaawqalga
      301 ragehglsaa gvalaafaet igrwsqapaf clnltvlnrp plhpqlaqvl gdftalslla
      361 vdsrhgdsfv erarrigeqm fddldhptfs gvdllrelar rrgrgadlmp vvftsgigsv
      421 qrllgdgeap raprymisqt pqvwldcqvt dqfggleigw dvrlglfpeg qaeamfddfv
      481 gllrrlaqsp rawtdgdate pveappqalp gsarsiaagf aeralltpda taihdaagsy
      541 syrqvaqhas alrrvleahg agrgrrvavm lpksaaqlva vigilqagaa yvpvdirqpp
      601 lrrqailasa evvalvcles dvpdvgcacv aidrlaadsa wppppaaeva addlayviyt
      661 sgstgtpkgv mlshaavsnt lldinqrygv dandrvlgla elsfdlsvyd ffgataagaq
      721 vvlpdpargs dpshwaelle rhaitlwnsv paqgqmlidy lesepqrhlp gprcvlwsgd
      781 wipvslptrw wrrwpdsalf slggateaai wsieqpirpq htelasipyg ralrgqsvev
      841 ldargrrcpp gvrgeihigg vglalgyagd pqrtaerfvr hpdgrrlyrt gdlgryladg
      901 sieflgredd qvkirghrie laeldaalca hpqvnlaatv vlgethersl asfvtlhapv
      961 eagedprtal davrqraaqa lrrdwgseeg iaaavaaldr aclaslaawl agsglfasat
     1021 pldlatlcqr lgiaearqrl lrhwlrqlee ggylraegeg wlgcaerpaq spedawtafa
     1081 gcapaalwpa elvaylrdsa qslgeqlagr ispaalmfpq gsariaeamy sqglhaqalh
     1141 eamaeaiaai verqpqrrwr llelgagtaa asrtviarla plvqrgaevd ylftdvssyf
     1201 laaarerfad qpwvrfgrfd mngdlldqgv aphsvdills sgalnnaldt pallaglrel
     1261 lsadawlviq eltrehneis vsqslmmenp rdlrderrql fvhtgqwlew laaqggdlac
     1321 gvvppgsald llgydvllar cktdrarlep aellafvear vprymlpaql rvlerlpvtg
     1381 ngkidrkalt gfarqpqadl rhgvaqapad elenallalw revldnpslg veqdffgagg
     1441 dslliaqlia rlrerlesar rhpfdrllrw alsqptprgl aerlrsapee grgpalaaar
     1501 gvapapagms raplaegava ldplvrlvpg egvprvlvhe glgtllpyrp llralgegrp
     1561 llglavhdsd aylaipaehl naclgrryae alhraglrev dllgycsggl valetakslv
     1621 qrgvrvrqld ivssyripyr vdderlllfs faatlgldta algfpaperl gqavqaalaq
     1681 tperlvaeal aglpgladlv alrgrvlqaa sgsadavsve rdtlyrlfch svrasqaeap
     1741 epyvgalrlf vpdagnplvp ryaealetqw raaalgacgi hevpgghfdc lgealaqsls
     1801 kpmpeeasr