MULTISPECIES: type B-4 chloramphenicol O-acetyltransferase CatB7

LOCUS       WP_003112709             212 aa            linear   BCT 20-NOV-2023
            [Pseudomonas].
ACCESSION   WP_003112709
VERSION     WP_003112709.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 212)
  AUTHORS   Alcala,A., Ramirez,G., Solis,A., Kim,Y., Tan,K., Luna,O.,
            Nguyen,K., Vazquez,D., Ward,M., Zhou,M., Mulligan,R., Maltseva,N.
            and Kuhn,M.L.
  TITLE     Structural and functional characterization of three Type B and C
            chloramphenicol acetyltransferases from Vibrio species
  JOURNAL   Protein Sci 29 (3), 695-710 (2020)
   PUBMED   31762145
REFERENCE   2  (residues 1 to 212)
  AUTHORS   Schwarz,S., Kehrenberg,C., Doublet,B. and Cloeckaert,A.
  TITLE     Molecular basis of bacterial resistance to chloramphenicol and
            florfenicol
  JOURNAL   FEMS Microbiol Rev 28 (5), 519-542 (2004)
   PUBMED   15539072
REFERENCE   3  (residues 1 to 212)
  AUTHORS   Parent,R. and Roy,P.H.
  TITLE     The chloramphenicol acetyltransferase gene of Tn2424: a new breed
            of cat
  JOURNAL   J Bacteriol 174 (9), 2891-2897 (1992)
   PUBMED   1314803
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Antimicrobial Resistance Protein
            Evidence Accession :: NG_047614.1
            Evidence Source    :: Bacterial Antimicrobial Resistance Reference
                                  Gene Database
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     gene            1..212
                     /gene="catB"
     Protein         1..212
                     /product="type B-4 chloramphenicol O-acetyltransferase
                     CatB7"
                     /EC_number="2.3.1.28"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /calculated_mol_wt=23394
     Region          46..182
                     /region_name="LbH_XAT"
                     /note="Xenobiotic acyltransferase (XAT): The XAT class of
                     hexapeptide acyltransferases is composed of a large number
                     of microbial enzymes that catalyze the CoA-dependent
                     acetylation of a variety of hydroxyl-bearing acceptors
                     such as chloramphenicol and...; cd03349"
                     /db_xref="CDD:100040"
     Site            order(46,64,79..80,85,87,89,116,118,121,140,156,164)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:100040"
     Site            order(46,48,76,79,81,90..91,118,121,138..139,144,152,
                     154..155,157,161)
                     /site_type="active"
                     /db_xref="CDD:100040"
     Site            order(46,48,76,90..91)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:100040"
     Site            order(79,81,108,118,121,126,138..139,144,152,154..155,157,
                     161)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:100040"
ORIGIN      
        1 mgnyfespfr gkllseqvsn pnirvgrysy ysgyyhghsf ddcarylmpd rddvdklvig
       61 sfcsigsgaa fimagnqghr aewastfpfh fmheepvfag avngyqpagd tlighdvwig
      121 teamfmpgvr vghgaiigsr alvtgdvepy aivggnpart irkrfsdgdi qnllemawwd
      181 wpladieaam pllctgdipa lyrhwkqrqa ta