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general PTS family enzyme I [Salmonella enterica subsp. enterica


LOCUS       NP_462991                833 aa            linear   CON 08-SEP-2022
            serovar Typhimurium str. LT2].
ACCESSION   NP_462991
VERSION     NP_462991.1
DBLINK      BioProject: PRJNA57799
            BioSample: SAMN02604315
DBSOURCE    REFSEQ: accession NC_003197.2
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 833)
  AUTHORS   McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W.,
            Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F.,
            Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A.,
            Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W.,
            Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K.
  TITLE     Complete genome sequence of Salmonella enterica serovar Typhimurium
            LT2
  JOURNAL   Nature 413 (6858), 852-856 (2001)
   PUBMED   11677609
REFERENCE   2  (residues 1 to 833)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (residues 1 to 833)
  AUTHORS   McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R.,
            SanMiguel,P. and Csonka,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2016) Department of Microbiology and Molecular
            Genetics, University of California, Irvine, CA 92697, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (residues 1 to 833)
  CONSRTM   The Salmonella typhimurium Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2001) Genome Sequencing Center, Department of
            Genetics, Washington University School of Medicine, 4444 Forest
            Park Boulevard, St. Louis, MO 63108, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAL22950.
            Supported by NIH grant 5U 01 AI43283
            
            Coding sequences below are predicted from manually evaluated
            computer analysis, using similarity information and the programs;
            GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and
            GeneMark; http://opal.biology.gatech.edu/GeneMark/
            
            EC numbers were kindly provided by Junko Yabuzaki and the Kyoto
            Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/,
            and Pedro Romero and Peter Karp at EcoCyc;
            http://ecocyc.PangeaSystems.com/ecocyc/
            
            The analyses of ribosome binding sites and promoter binding sites
            were kindly provided by Heladia Salgado, Julio Collado-Vides and
            ReguonDB;
            http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset
            
            This sequence was finished as follows unless otherwise noted: all
            regions were double stranded, sequenced with an alternate
            chemistries or covered by high quality data (i.e., phred quality >=
            30); an attempt was made to resolve all sequencing problems, such
            as compressions and repeats; all regions were covered by sequence
            from more than one m13 subclone.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. LT2"
                     /strain="LT2"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /culture_collection="ATCC:700720"
                     /culture_collection="SGSC:1412"
                     /type_material="type strain of Salmonella enterica"
                     /db_xref="taxon:99287"
                     /focus
     Protein         1..833
                     /product="general PTS family enzyme I"
                     /EC_number="2.7.3.9"
                     /calculated_mol_wt=91952
     Region          6..84
                     /region_name="PTS-HPr_like"
                     /note="Histidine-containing phosphocarrier protein
                     (HPr)-like proteins. HPr is a central component of the
                     bacterial phosphoenolpyruvate sugar phosphotransferase
                     system (PTS). The PTS catalyses the phosphorylation of
                     sugar substrates during their translocation...; cd00367"
                     /db_xref="CDD:238217"
     Site            6..9
                     /site_type="other"
                     /note="dimerization domain swap beta strand [polypeptide
                     binding]"
                     /db_xref="CDD:238217"
     Site            order(15,17..18,20..21,25,28,48..51,54..55)
                     /site_type="other"
                     /note="regulatory protein interface [polypeptide binding]"
                     /db_xref="CDD:238217"
     Site            16
                     /site_type="active"
                     /db_xref="CDD:238217"
     Site            49
                     /site_type="other"
                     /note="regulatory phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:238217"
     Region          118..684
                     /region_name="PtsA"
                     /note="Phosphoenolpyruvate-protein kinase (PTS system EI
                     component in bacteria) [Carbohydrate transport and
                     metabolism]; COG1080"
                     /db_xref="CDD:440698"
     Region          687..831
                     /region_name="PtsN"
                     /note="Phosphotransferase system
                     mannitol/fructose-specific IIA domain (Ntr-type)
                     [Carbohydrate transport and metabolism, Signal
                     transduction mechanisms]; COG1762"
                     /db_xref="CDD:441368"
     CDS             1..833
                     /gene="ptsA"
                     /locus_tag="STM4110"
                     /coded_by="complement(NC_003197.2:4324504..4327005)"
                     /note="similar to E. coli PEP-protein phosphotransferase
                     system enzyme I (AAC76929.1); Blastp hit to AAC76929.1
                     (711 aa), 90% identity in aa 1 - 711"
                     /transl_table=11
                     /db_xref="GeneID:1255637"
CONTIG      join(WP_001128495.1:1..833)
ORIGIN      
        1 mpliveftce lpngvharpa shvetlcntf tsqiewhnlr tdrkgsaksa laligtdtla
       61 gdhcqlvisg adeqvacqrl sqwlrdefpl cdaplaeikn seleplpasl tqlnpqiyra
      121 rsvcsgsagg vltplssldl nalgelptan dteteqaald nglamlikhi efrqldsdga
      181 asaileahrs lagdaslrqh lldgvlrgls caqaivesan hfcnefaras ssylqerald
      241 vrdvcfqllq hiyggqrfpa pgqltrpsic maeeltpsqf leldktflkg lllksggtts
      301 htvilarsfn iptlvgveie altpwrqqtv yidgnagaiv vapdepvtry yqqearvqda
      361 lreqqriwlt qeartadgir mevaaniahs veaqaafsng aeavglfrte mlymdracap
      421 denelynifc qalesakgrs iivrtmdigg dkpvdylnip aeanpflgyr avriyeeyas
      481 lfttqlrsil rasahgnlki mipmissmee ilwvkeklae akqqlrnehi pfdekiplgi
      541 mlevpsvmfi idqcceeidf fsigsndltq yllavdrdna kvtrhynsln paflraldfa
      601 vqavhrqgkw iglcgelgak gsvlpllvgl gldeismgap sipaakarma qldsracrql
      661 lnqamacrts levehllaqf rmsqqdaplv taqcitldsd wrskeevikg mtdnlllagr
      721 cryprklead lwareavfst glgfsfaiph sksehieqst isvarlnapv rwgddeaqfi
      781 imltlnkhaa gdqhmrifsr larrimheef rntlvnaasa daiasllqhe lel