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LOCUS NP_462155 764 aa linear CON 08-SEP-2022 serovar Typhimurium str. LT2]. ACCESSION NP_462155 VERSION NP_462155.1 DBLINK BioProject: PRJNA57799 BioSample: SAMN02604315 DBSOURCE REFSEQ: accession NC_003197.2 KEYWORDS RefSeq. SOURCE Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ORGANISM Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella. REFERENCE 1 (residues 1 to 764) AUTHORS McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W., Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F., Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A., Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W., Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K. TITLE Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 JOURNAL Nature 413 (6858), 852-856 (2001) PUBMED 11677609 REFERENCE 2 (residues 1 to 764) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (08-SEP-2022) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (residues 1 to 764) AUTHORS McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R., SanMiguel,P. and Csonka,L. TITLE Direct Submission JOURNAL Submitted (13-JAN-2016) Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697, USA REMARK Sequence update by submitter REFERENCE 4 (residues 1 to 764) CONSRTM The Salmonella typhimurium Genome Sequencing Project TITLE Direct Submission JOURNAL Submitted (29-MAR-2001) Genome Sequencing Center, Department of Genetics, Washington University School of Medicine, 4444 Forest Park Boulevard, St. Louis, MO 63108, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AAL22114. Supported by NIH grant 5U 01 AI43283 Coding sequences below are predicted from manually evaluated computer analysis, using similarity information and the programs; GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and GeneMark; http://opal.biology.gatech.edu/GeneMark/ EC numbers were kindly provided by Junko Yabuzaki and the Kyoto Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/, and Pedro Romero and Peter Karp at EcoCyc; http://ecocyc.PangeaSystems.com/ecocyc/ The analyses of ribosome binding sites and promoter binding sites were kindly provided by Heladia Salgado, Julio Collado-Vides and ReguonDB; http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by sequence from more than one m13 subclone. Method: conceptual translation. FEATURES Location/Qualifiers source 1..764 /organism="Salmonella enterica subsp. enterica serovar Typhimurium str. LT2" /strain="LT2" /serovar="Typhimurium" /sub_species="enterica" /culture_collection="ATCC:700720" /culture_collection="SGSC:1412" /type_material="type strain of Salmonella enterica" /db_xref="taxon:99287" /focus Protein 1..764 /product="2-ketobutyrate formate-lyase" /EC_number="2.3.1.54" /note="pyruvate formate-lyase 4" /calculated_mol_wt=85734 Region 15..760 /region_name="PFL1" /note="Pyruvate formate lyase 1; cd01678" /db_xref="CDD:153087" Site order(121..122,150..151,154..155,157,162,165..166) /site_type="other" /note="coenzyme A binding site [chemical binding]" /db_xref="CDD:153087" Site order(181,277..278,332,338,423,437,440,609,611) /site_type="active" /db_xref="CDD:153087" Site order(423..424,739) /site_type="active" /note="catalytic residues [active]" /db_xref="CDD:153087" Site 736..740 /site_type="active" /note="glycine loop [active]" /db_xref="CDD:153087" CDS 1..764 /gene="tdcE" /locus_tag="STM3241" /coded_by="complement(NC_003197.2:3406439..3408733)" /note="similar to E. coli probable formate acetyltransferase 3 (AAC76149.1); Blastp hit to AAC76149.1 (746 aa), 93% identity in aa 1 - 741" /transl_table=11 /db_xref="GeneID:1254764" CONTIG join(WP_000867323.1:1..764) ORIGIN 1 mkvnidtsdm lyaeawrdfk gtdwkeeinv rdfiqhnytp yegdesflad atpattalwe 61 kvmagirien athapvdfdt niattitahd agyiekelek ivglqtdkpl kralhpfggv 121 nmikssfhay gremdadfey tftdlrkthn qgvfdvyspd mlrcrksgvl tglpdgygrg 181 riigdyrrva lygirylvre relqfadlqs nleqgqnlea tirlreelae hrrallqmqe 241 maakygydis rparnaqeav qwlyfaylaa vksqnggams lgrtasfldi yierdfnagl 301 lteqqaqeli dhfimkirmv rflrtpefds lfsgdpiwat eviggmgldg rtlvtknsfr 361 ylhtlhtmgp apepnltilw sealpvafkk yaaqvsivts slqyenddlm rtdfnsddya 421 iaccvspmvi gkqmqffgar anlaktllya inggvdeklk iqvgpktapl tdevldydav 481 mesldhfmdw lavqyisaln iihymhdkys yeaslmalhd rdvyrtmacg iaglsvaads 541 lsairyaqvk pirdenglai dfaiegeypq ygnndervds iacdlvkrfm qkisvlptyr 601 navptqsilt itsnvvygqk tgntpdgrra gtpfapganp mhgrdrkgav asltsvaklp 661 ftyakdgisy tfsivpaalg kedavrktnl vglldgyfhh eaqveggqhl nvnvmnreml 721 ldaiehpeny pnltirvsgy avrfnaltre qqqdvisrtf tqam