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LOCUS NP_461929 340 aa linear CON 08-SEP-2022 enterica serovar Typhimurium str. LT2]. ACCESSION NP_461929 VERSION NP_461929.1 DBLINK BioProject: PRJNA57799 BioSample: SAMN02604315 DBSOURCE REFSEQ: accession NC_003197.2 KEYWORDS RefSeq. SOURCE Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ORGANISM Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella. REFERENCE 1 (residues 1 to 340) AUTHORS McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W., Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F., Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A., Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W., Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K. TITLE Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 JOURNAL Nature 413 (6858), 852-856 (2001) PUBMED 11677609 REFERENCE 2 (residues 1 to 340) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (08-SEP-2022) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (residues 1 to 340) AUTHORS McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R., SanMiguel,P. and Csonka,L. TITLE Direct Submission JOURNAL Submitted (13-JAN-2016) Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697, USA REMARK Sequence update by submitter REFERENCE 4 (residues 1 to 340) CONSRTM The Salmonella typhimurium Genome Sequencing Project TITLE Direct Submission JOURNAL Submitted (29-MAR-2001) Genome Sequencing Center, Department of Genetics, Washington University School of Medicine, 4444 Forest Park Boulevard, St. Louis, MO 63108, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AAL21888. Supported by NIH grant 5U 01 AI43283 Coding sequences below are predicted from manually evaluated computer analysis, using similarity information and the programs; GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and GeneMark; http://opal.biology.gatech.edu/GeneMark/ EC numbers were kindly provided by Junko Yabuzaki and the Kyoto Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/, and Pedro Romero and Peter Karp at EcoCyc; http://ecocyc.PangeaSystems.com/ecocyc/ The analyses of ribosome binding sites and promoter binding sites were kindly provided by Heladia Salgado, Julio Collado-Vides and ReguonDB; http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by sequence from more than one m13 subclone. Method: conceptual translation. FEATURES Location/Qualifiers source 1..340 /organism="Salmonella enterica subsp. enterica serovar Typhimurium str. LT2" /strain="LT2" /serovar="Typhimurium" /sub_species="enterica" /culture_collection="ATCC:700720" /culture_collection="SGSC:1412" /type_material="type strain of Salmonella enterica" /db_xref="taxon:99287" /focus Protein 1..340 /product="putative transcriptional regulator" /calculated_mol_wt=37175 Region 3..71 /region_name="HTH_LACI" /note="helix_turn _helix lactose operon repressor; smart00354" /db_xref="CDD:197675" Site order(14..18,20..21,24,29..30,33,46,49,52..53,55..56) /site_type="DNA binding" /note="DNA binding site [nucleotide binding]" /db_xref="CDD:143331" Site 46..55 /site_type="active" /note="domain linker motif [active]" /db_xref="CDD:143331" Region 62..328 /region_name="PBP1_GalS-like" /note="ligand binding domain of DNA transcription iso-repressor GalS, which is one of two regulatory proteins involved in galactose transport and metabolism; cd06270" /db_xref="CDD:380494" Site order(62,69,75,80,83,92..95,97,111,115,223..224,256, 282..283) /site_type="other" /note="dimer interface (closed form) [polypeptide binding]" /db_xref="CDD:380494" Site order(74,123,196,221,247,275) /site_type="other" /note="ligand binding site [chemical binding]" /db_xref="CDD:380494" CDS 1..340 /locus_tag="STM3012" /coded_by="NC_003197.2:3171669..3172691" /note="similar to E. coli ascBF operon repressor (AAC75756.1); Blastp hit to AAC75756.1 (337 aa), 41% identity in aa 1 - 334" /transl_table=11 /db_xref="GeneID:1254535" CONTIG join(WP_000968354.1:1..340) ORIGIN 1 mmatmldvsr hagvskatvs rvlngtgqvk estrqkvfta mqalgyrpnl larslanrts 61 nsiglvvstf dgfyfgsllr rasrqaeshn kqlivtdghd tpereqkavq mladrqcdai 121 ilytrymdel ailslidate mplviinrnv tqardraiff eqetaafqav eylitqghrd 181 iacitlpvht ptgtsrvagy rkalekygip wqpakvkygd ytltrgydac relleegvtf 241 salfacnddt algaakalrq aglripqdvs lfgfddapga twlepglstv ylpieamiat 301 aidqavrlan sepvapippf tgtlilresv aagpffqrpa