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RND family aminoglycoside/multidrug efflux pump [Salmonella


LOCUS       NP_461416               1037 aa            linear   CON 08-SEP-2022
            enterica subsp. enterica serovar Typhimurium str. LT2].
ACCESSION   NP_461416
VERSION     NP_461416.1
DBLINK      BioProject: PRJNA57799
            BioSample: SAMN02604315
DBSOURCE    REFSEQ: accession NC_003197.2
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 1037)
  AUTHORS   McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W.,
            Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F.,
            Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A.,
            Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W.,
            Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K.
  TITLE     Complete genome sequence of Salmonella enterica serovar Typhimurium
            LT2
  JOURNAL   Nature 413 (6858), 852-856 (2001)
   PUBMED   11677609
REFERENCE   2  (residues 1 to 1037)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (residues 1 to 1037)
  AUTHORS   McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R.,
            SanMiguel,P. and Csonka,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2016) Department of Microbiology and Molecular
            Genetics, University of California, Irvine, CA 92697, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (residues 1 to 1037)
  CONSRTM   The Salmonella typhimurium Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2001) Genome Sequencing Center, Department of
            Genetics, Washington University School of Medicine, 4444 Forest
            Park Boulevard, St. Louis, MO 63108, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAL21375.
            Supported by NIH grant 5U 01 AI43283
            
            Coding sequences below are predicted from manually evaluated
            computer analysis, using similarity information and the programs;
            GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and
            GeneMark; http://opal.biology.gatech.edu/GeneMark/
            
            EC numbers were kindly provided by Junko Yabuzaki and the Kyoto
            Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/,
            and Pedro Romero and Peter Karp at EcoCyc;
            http://ecocyc.PangeaSystems.com/ecocyc/
            
            The analyses of ribosome binding sites and promoter binding sites
            were kindly provided by Heladia Salgado, Julio Collado-Vides and
            ReguonDB;
            http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset
            
            This sequence was finished as follows unless otherwise noted: all
            regions were double stranded, sequenced with an alternate
            chemistries or covered by high quality data (i.e., phred quality >=
            30); an attempt was made to resolve all sequencing problems, such
            as compressions and repeats; all regions were covered by sequence
            from more than one m13 subclone.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. LT2"
                     /strain="LT2"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /culture_collection="ATCC:700720"
                     /culture_collection="SGSC:1412"
                     /type_material="type strain of Salmonella enterica"
                     /db_xref="taxon:99287"
                     /focus
     Protein         1..1037
                     /product="RND family aminoglycoside/multidrug efflux pump"
                     /calculated_mol_wt=112821
     Region          1..1036
                     /region_name="PRK10555"
                     /note="multidrug efflux RND transporter permease AcrD"
                     /db_xref="CDD:182544"
     CDS             1..1037
                     /gene="acrD"
                     /locus_tag="STM2481"
                     /coded_by="NC_003197.2:2593646..2596759"
                     /note="similar to E. coli sensitivity to acriflavine,
                     integral membrane protein, possible efflux pump
                     (AAC75523.1); Blastp hit to AAC75523.1 (1037 aa), 94%
                     identity in aa 1 - 1037"
                     /transl_table=11
                     /db_xref="GeneID:1254003"
CONTIG      join(WP_001263078.1:1..1037)
ORIGIN      
        1 manffidrpi fawvlaillc ltgalaifsl pveqypdlap pnvritanyp gasaqtlent
       61 vtqvieqnmt gldnlmymss qssgtgqati tlsfiagtdp deavqqvqnq lqsamrklpq
      121 avqdqgvtvr ktgdtnilti afvstdgsmd kqdiadyvas niqdplsrvn gvgdidaygs
      181 qysmriwldp aklnsfqmtt kdvtdaiesq naqiavgqlg gtpsvdkqal natinaqsll
      241 qtpqqfrdit lrvnqdgsev klgdvatvel gaekydylsr fngnpasglg vklasganem
      301 ataklvldrl nelaqyfphg leykiayett sfvkasiidv vktlleaial vflvmylflq
      361 nfratlipti avpvvlmgtf svlyafgysi ntltmfamvl aigllvddai vvvenverim
      421 seegltprea trksmgqiqg alvgiamvls avfvpmaffg gttgaiyrqf sitivsamvl
      481 svlvamiltp alcatllkpl hkgeqhgqrg ffgwfnrtfn rnaeryekgv akilhrslrw
      541 iliyvlllgg mvflflrlpt sflpqedrgm fttsiqlpsg stqqqtlkvv ekvenyyfth
      601 ekdnimsvfs tvgsgpggng qnvarmfvrl kdwdardptt gssfaiiera tkafnqikea
      661 rvfassppai sglgssagfd melqdhagag hdalmaardq lielagknss ltrvrhngld
      721 dspqlqidid qrkaqalgvs iddindtlqt awgssyvndf mdrgrvkkvy vqaaakyrml
      781 pddinlwyvr nkdggmvpfs afatsrwetg sprleryngy saveivgeaa pgvstgtamd
      841 vmeslvhqlp ggfglewtam syqerlsgaq apalyaisll vvflclaaly eswsvpfsvm
      901 lvvplgviga llatwmrgle ndvyfqvgll tviglsakna ilivefanem nqkghallda
      961 tlyasrqrlr pilmtslafi fgvlpmatst gagsgsqhav gtgvmggmis atvlaiffvp
     1021 lffvlirrrf plkprpk