putative dehydratase protein [Salmonella enterica subsp. enterica

LOCUS       NP_461215                400 aa            linear   CON 08-SEP-2022
            serovar Typhimurium str. LT2].
ACCESSION   NP_461215
VERSION     NP_461215.1
DBLINK      BioProject: PRJNA57799
            BioSample: SAMN02604315
DBSOURCE    REFSEQ: accession NC_003197.2
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 400)
  AUTHORS   McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W.,
            Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F.,
            Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A.,
            Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W.,
            Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K.
  TITLE     Complete genome sequence of Salmonella enterica serovar Typhimurium
            LT2
  JOURNAL   Nature 413 (6858), 852-856 (2001)
   PUBMED   11677609
REFERENCE   2  (residues 1 to 400)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (residues 1 to 400)
  AUTHORS   McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R.,
            SanMiguel,P. and Csonka,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2016) Department of Microbiology and Molecular
            Genetics, University of California, Irvine, CA 92697, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (residues 1 to 400)
  CONSRTM   The Salmonella typhimurium Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2001) Genome Sequencing Center, Department of
            Genetics, Washington University School of Medicine, 4444 Forest
            Park Boulevard, St. Louis, MO 63108, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAL21174.
            Supported by NIH grant 5U 01 AI43283
            
            Coding sequences below are predicted from manually evaluated
            computer analysis, using similarity information and the programs;
            GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and
            GeneMark; http://opal.biology.gatech.edu/GeneMark/
            
            EC numbers were kindly provided by Junko Yabuzaki and the Kyoto
            Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/,
            and Pedro Romero and Peter Karp at EcoCyc;
            http://ecocyc.PangeaSystems.com/ecocyc/
            
            The analyses of ribosome binding sites and promoter binding sites
            were kindly provided by Heladia Salgado, Julio Collado-Vides and
            ReguonDB;
            http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset
            
            This sequence was finished as follows unless otherwise noted: all
            regions were double stranded, sequenced with an alternate
            chemistries or covered by high quality data (i.e., phred quality >=
            30); an attempt was made to resolve all sequencing problems, such
            as compressions and repeats; all regions were covered by sequence
            from more than one m13 subclone.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. LT2"
                     /strain="LT2"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /culture_collection="ATCC:700720"
                     /culture_collection="SGSC:1412"
                     /type_material="type strain of Salmonella enterica"
                     /db_xref="taxon:99287"
                     /focus
     Protein         1..400
                     /product="putative dehydratase protein"
                     /calculated_mol_wt=44316
     Region          2..385
                     /region_name="MR_like"
                     /note="Mandelate racemase (MR)-like subfamily of the
                     enolase superfamily. Enzymes of this subgroup share three
                     conserved carboxylate ligands for the essential divalent
                     metal ion (usually Mg2+), two aspartates and a glutamate,
                     and conserved catalytic residues; cd03316"
                     /db_xref="CDD:239432"
     Site            order(168,170,222,248,274,297,324,349)
                     /site_type="active"
                     /note="active site pocket [active]"
                     /db_xref="CDD:239432"
     CDS             1..400
                     /locus_tag="STM2273"
                     /coded_by="complement(NC_003197.2:2376756..2377958)"
                     /note="similar to E. coli starvation sensing protein
                     (AAC74653.1); Blastp hit to AAC74653.1 (404 aa), 30%
                     identity in aa 198 - 384, 36% identity in aa 1 - 120"
                     /transl_table=11
                     /db_xref="GeneID:1253795"
CONTIG      join(WP_000705579.1:1..400)
ORIGIN      
        1 mkitsievfd celkkrdqtm ssynpvlirv ntdsglsgig evglaygaga kagvgiirdl
       61 aplivgedpl niekiwefff rktfwgmggg nvfyagmsai dialwdikgk ylgvpvyqll
      121 ggktneklrt yasqlqfgwg dkrhilvtpe eyaeaaraal ddgydaikvd pleidrngdd
      181 cvfqnrnrny sgllladqlk mgeariaamr eamgddadii veihsllgtn saiqfakaie
      241 kyriflyeep ihplnsdnmq kvsrsttipi atgersytrw gyrellekqs iavaqpdlcl
      301 cggitegkki cdyaniydtt vqvhvcggpv stvaalhmet aipnfiiheh htnamkasir
      361 elcthdyqpe ngyyvapeqp glgqelndev vkeylayvik