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periplasmic large subunit nitrate reductase [Salmonella enterica


LOCUS       NP_461202                828 aa            linear   CON 08-SEP-2022
            subsp. enterica serovar Typhimurium str. LT2].
ACCESSION   NP_461202
VERSION     NP_461202.1
DBLINK      BioProject: PRJNA57799
            BioSample: SAMN02604315
DBSOURCE    REFSEQ: accession NC_003197.2
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 828)
  AUTHORS   McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W.,
            Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F.,
            Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A.,
            Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W.,
            Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K.
  TITLE     Complete genome sequence of Salmonella enterica serovar Typhimurium
            LT2
  JOURNAL   Nature 413 (6858), 852-856 (2001)
   PUBMED   11677609
REFERENCE   2  (residues 1 to 828)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (residues 1 to 828)
  AUTHORS   McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R.,
            SanMiguel,P. and Csonka,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2016) Department of Microbiology and Molecular
            Genetics, University of California, Irvine, CA 92697, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (residues 1 to 828)
  CONSRTM   The Salmonella typhimurium Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2001) Genome Sequencing Center, Department of
            Genetics, Washington University School of Medicine, 4444 Forest
            Park Boulevard, St. Louis, MO 63108, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAL21161.
            Supported by NIH grant 5U 01 AI43283
            
            Coding sequences below are predicted from manually evaluated
            computer analysis, using similarity information and the programs;
            GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and
            GeneMark; http://opal.biology.gatech.edu/GeneMark/
            
            EC numbers were kindly provided by Junko Yabuzaki and the Kyoto
            Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/,
            and Pedro Romero and Peter Karp at EcoCyc;
            http://ecocyc.PangeaSystems.com/ecocyc/
            
            The analyses of ribosome binding sites and promoter binding sites
            were kindly provided by Heladia Salgado, Julio Collado-Vides and
            ReguonDB;
            http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset
            
            This sequence was finished as follows unless otherwise noted: all
            regions were double stranded, sequenced with an alternate
            chemistries or covered by high quality data (i.e., phred quality >=
            30); an attempt was made to resolve all sequencing problems, such
            as compressions and repeats; all regions were covered by sequence
            from more than one m13 subclone.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. LT2"
                     /strain="LT2"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /culture_collection="ATCC:700720"
                     /culture_collection="SGSC:1412"
                     /type_material="type strain of Salmonella enterica"
                     /db_xref="taxon:99287"
                     /focus
     Protein         1..828
                     /product="periplasmic large subunit nitrate reductase"
                     /EC_number="1.7.99.4"
                     /calculated_mol_wt=92743
     Region          1..828
                     /region_name="PRK13532"
                     /note="nitrate reductase catalytic subunit NapA"
                     /db_xref="CDD:237416"
     Site            order(46,48..49,53,81,222)
                     /site_type="other"
                     /note="[4Fe-4S] binding site [ion binding]"
                     /db_xref="CDD:239155"
     Site            order(83,150,175,179,212..215,218..219,243..245,262,264,
                     371..373,376,409..410,481..482,486,508..511,525..527,531,
                     558)
                     /site_type="other"
                     /note="molybdopterin cofactor binding site"
                     /db_xref="CDD:239155"
     CDS             1..828
                     /gene="napA"
                     /locus_tag="STM2259"
                     /coded_by="complement(NC_003197.2:2356417..2358903)"
                     /note="complexes with NapB; similar to E. coli probable
                     nitrate reductase 3 (AAC75266.1); Blastp hit to AAC75266.1
                     (828 aa), 94% identity in aa 1 - 828"
                     /transl_table=11
                     /db_xref="GeneID:1253781"
CONTIG      join(WP_000778097.1:1..828)
ORIGIN      
        1 mklsrrsfmk anavaaaaaa aglsvpgvar avvgqqeaik wdkapcrfcg tgcgvlvgtq
       61 qgrvvacqgd pdapvnrgln cikgyflpki mygkdrltqp mlrmkdgsyh kdgeftpvsw
      121 eqafdvmeek fktslkekgp eaigmfgsgq wtiwegyaaa klfkagfrsn nidpnarhcm
      181 asavvgfmrt fgmdepmgcy ddieqadafv lwgsnmaemh pilwsritnr rlsdpnvkva
      241 vlstfqhrsf eladngivft pqsdlvilny ianyiiqnna vnqdfftkhv nlrkgatdig
      301 yglrpthple kaaknpgsda sepmsfdeyk afvaeytldk taemtgvpkd qleqlaqlya
      361 dpnkrvisyw tmgfnqhtrg vwannlvynl hlltgkisqp gcgpfsltgq psacgtarev
      421 gtfshrlpad mvvtnekhrd icekhwqipa gtipakvglh avaqdralkd gklnvywvmc
      481 nnnmqagpni nedrmpgwrd prnfiivsdp yptvsalsad lilptamwve kegaygnaer
      541 rtqfwrqqik apgeaksdlw qlvqfsrrfk teevwpeall aqkpelrgkt lydvlfatpa
      601 vskfplselk edqlndesre lgfylqkglf eeyawfgrgh ghdlapfddy hnarglrwpv
      661 vegketqwry segndpyvka gegykfygkp dgkavifalp fepaaespdn eydlwlstgr
      721 vlehwhtgsm trrvpelhra fpeavvfihp ldakardlrr gdkvkvssrr gevisivetr
      781 grnrppqglv ympffdaaql vnnltldatd plsketdfkk cavklakv