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LOCUS NP_459948 760 aa linear CON 08-SEP-2022 enterica subsp. enterica serovar Typhimurium str. LT2]. ACCESSION NP_459948 VERSION NP_459948.1 DBLINK BioProject: PRJNA57799 BioSample: SAMN02604315 DBSOURCE REFSEQ: accession NC_003197.2 KEYWORDS RefSeq. SOURCE Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ORGANISM Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella. REFERENCE 1 (residues 1 to 760) AUTHORS McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W., Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F., Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A., Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W., Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K. TITLE Complete genome sequence of Salmonella enterica serovar Typhimurium LT2 JOURNAL Nature 413 (6858), 852-856 (2001) PUBMED 11677609 REFERENCE 2 (residues 1 to 760) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (08-SEP-2022) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (residues 1 to 760) AUTHORS McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R., SanMiguel,P. and Csonka,L. TITLE Direct Submission JOURNAL Submitted (13-JAN-2016) Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697, USA REMARK Sequence update by submitter REFERENCE 4 (residues 1 to 760) CONSRTM The Salmonella typhimurium Genome Sequencing Project TITLE Direct Submission JOURNAL Submitted (29-MAR-2001) Genome Sequencing Center, Department of Genetics, Washington University School of Medicine, 4444 Forest Park Boulevard, St. Louis, MO 63108, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AAL19907. Supported by NIH grant 5U 01 AI43283 Coding sequences below are predicted from manually evaluated computer analysis, using similarity information and the programs; GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and GeneMark; http://opal.biology.gatech.edu/GeneMark/ EC numbers were kindly provided by Junko Yabuzaki and the Kyoto Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/, and Pedro Romero and Peter Karp at EcoCyc; http://ecocyc.PangeaSystems.com/ecocyc/ The analyses of ribosome binding sites and promoter binding sites were kindly provided by Heladia Salgado, Julio Collado-Vides and ReguonDB; http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by sequence from more than one m13 subclone. Method: conceptual translation. FEATURES Location/Qualifiers source 1..760 /organism="Salmonella enterica subsp. enterica serovar Typhimurium str. LT2" /strain="LT2" /serovar="Typhimurium" /sub_species="enterica" /culture_collection="ATCC:700720" /culture_collection="SGSC:1412" /type_material="type strain of Salmonella enterica" /db_xref="taxon:99287" /focus Protein 1..760 /product="pyruvate formate lyase I, induced anaerobically" /EC_number="2.3.1.54" /calculated_mol_wt=84874 Region 11..756 /region_name="PFL1" /note="Pyruvate formate lyase 1; cd01678" /db_xref="CDD:153087" Site order(117..118,146..147,150..151,153,158,161..162) /site_type="other" /note="coenzyme A binding site [chemical binding]" /db_xref="CDD:153087" Site order(177,273..274,328,334,419,433,436,605,607) /site_type="active" /db_xref="CDD:153087" Site order(419..420,735) /site_type="active" /note="catalytic residues [active]" /db_xref="CDD:153087" Site 732..736 /site_type="active" /note="glycine loop [active]" /db_xref="CDD:153087" CDS 1..760 /gene="pflB" /locus_tag="STM0973" /coded_by="complement(NC_003197.2:1055099..1057381)" /note="similar to E. coli formate acetyltransferase 1 (AAC73989.1); Blastp hit to AAC73989.1 (760 aa), 96% identity in aa 1 - 760" /transl_table=11 /db_xref="GeneID:1252491" CONTIG join(WP_001292799.1:1..760) ORIGIN 1 mselneklat awegftkgdw qnevnvrdfi qknytpyegd esflagatda ttklwdsvme 61 gvkqenrtha pvdfdtsvas titshdagyi nkalekivgl qteaplkrai ipfggikmve 121 gsckaynrel dpmikkifte yrkthnqgvf dvytpdilrc rksgvltglp daygrgriig 181 dyrrvalygi dylmkdkfaq ftslqsdlen gvnleatirl reeiaeqhra lgqikemaak 241 ygcdisgpat naqeaiqwty fgylaavksq ngaamsfgrv stfldayier dlkagkiteq 301 daqemidhlv mklrmvrflr tpeydelfsg dpiwatesig gmgvdgrtlv tknsfrflnt 361 lytmgpspep nitvlwsekl plnfkkfaak vsidtsslqy enddlmrpdf nnddyaiacc 421 vspmivgkqm qffgaranla ktmlyaingg vdeklkmqvg pksepikgdv lnfdevmdrm 481 dhfmdwlakq yvtalnvihy mhdkysyeas lmalhdrdvi rtmacgiagl svaadslsai 541 kyakvkpird edglaidfei egeypqfgnn darvddmavd lverfmkkiq klttyrgaip 601 tqsvltitsn vvygkktgnt pdgrragapf gpganpmhgr dqkgavaslt svaklpfaya 661 kdgisytfsi vpnalgkdde vrktnlaglm dgyfhheasi eggqhlnvnv mnremlldam 721 ehpekypqlt irvsgyavrf nsltkeqqqd vitrtftqtm