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putative small-conductance mechanosensitive channel [Salmonella


LOCUS       NP_459473               1120 aa            linear   CON 08-SEP-2022
            enterica subsp. enterica serovar Typhimurium str. LT2].
ACCESSION   NP_459473
VERSION     NP_459473.1
DBLINK      BioProject: PRJNA57799
            BioSample: SAMN02604315
DBSOURCE    REFSEQ: accession NC_003197.2
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 1120)
  AUTHORS   McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W.,
            Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F.,
            Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A.,
            Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W.,
            Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K.
  TITLE     Complete genome sequence of Salmonella enterica serovar Typhimurium
            LT2
  JOURNAL   Nature 413 (6858), 852-856 (2001)
   PUBMED   11677609
REFERENCE   2  (residues 1 to 1120)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (residues 1 to 1120)
  AUTHORS   McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R.,
            SanMiguel,P. and Csonka,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2016) Department of Microbiology and Molecular
            Genetics, University of California, Irvine, CA 92697, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (residues 1 to 1120)
  CONSRTM   The Salmonella typhimurium Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2001) Genome Sequencing Center, Department of
            Genetics, Washington University School of Medicine, 4444 Forest
            Park Boulevard, St. Louis, MO 63108, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAL19432.
            Supported by NIH grant 5U 01 AI43283
            
            Coding sequences below are predicted from manually evaluated
            computer analysis, using similarity information and the programs;
            GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and
            GeneMark; http://opal.biology.gatech.edu/GeneMark/
            
            EC numbers were kindly provided by Junko Yabuzaki and the Kyoto
            Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/,
            and Pedro Romero and Peter Karp at EcoCyc;
            http://ecocyc.PangeaSystems.com/ecocyc/
            
            The analyses of ribosome binding sites and promoter binding sites
            were kindly provided by Heladia Salgado, Julio Collado-Vides and
            ReguonDB;
            http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset
            
            This sequence was finished as follows unless otherwise noted: all
            regions were double stranded, sequenced with an alternate
            chemistries or covered by high quality data (i.e., phred quality >=
            30); an attempt was made to resolve all sequencing problems, such
            as compressions and repeats; all regions were covered by sequence
            from more than one m13 subclone.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. LT2"
                     /strain="LT2"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /culture_collection="ATCC:700720"
                     /culture_collection="SGSC:1412"
                     /type_material="type strain of Salmonella enterica"
                     /db_xref="taxon:99287"
                     /focus
     Protein         1..1120
                     /product="putative small-conductance mechanosensitive
                     channel"
                     /calculated_mol_wt=126617
     Region          7..1120
                     /region_name="PRK11281"
                     /note="mechanosensitive channel MscK"
                     /db_xref="CDD:236892"
     CDS             1..1120
                     /gene="aefA"
                     /locus_tag="STM0478"
                     /coded_by="NC_003197.2:534529..537891"
                     /note="similar to E. coli putative alpha helix protein
                     (AAC73567.1); Blastp hit to AAC73567.1 (1120 aa), 89%
                     identity in aa 1 - 1117"
                     /transl_table=11
                     /db_xref="GeneID:1251998"
CONTIG      join(WP_000178249.1:1..1120)
ORIGIN      
        1 mtmlqlykrs qhfvfitisv liillscqsl afargqtngd lpskadvqnq ldtlnkqkdl
       61 saqdklvqqd lidtlatlek iervkeetvq lrqkvaqape kmrqataaln alsdvdndde
      121 mrktlsalsl rqlelrvaqv lddlqnsqnd laaynsqlvs lqtqpervqn amytasqqiq
      181 qirnrldgnn vgeaalrpsq qvllqaqqal lnaqidqqrk slegntvlqd tlqkqrdyvt
      241 ansnrlehql qllqeavnsk rltltektaq eaispdetar iqanplvkqe ldinhqlsqr
      301 livatengnm lmqqnikvkn wldralqser nikeqiavlk gslllsrily qqqqtlpsad
      361 eledmtnria dlrleqfein qqrdalfqsd afvdkleegh tsevndevhd allqvvemrr
      421 elldqlnkql gnqlmmainl qvnqqqlmsv sknlkailtq qifwvnsnrp mdwdwlkafp
      481 qtlkeqfsam kitvnwqkaw pavfiaflag lpllliagli rwrlkwlkay qqklaaavgs
      541 lrndsqlntp kailidlira lpvcliilal glilltmqln isdllwafsk klamfwlvfg
      601 lcwkvlekeg vairhfgmpa qltshwrrqi vrislallpl hfwsvvaels plnlmddvlg
      661 qaviflnllv itllvwplcr eswrdkeshg irlvtvtils iipvalmvlt atgyfyttlr
      721 lagrwietvy lviiwnllyq tvlrglsvaa rriawrrala rrqnlvkega egaepqeept
      781 ialeqinqqt lritmllmla lfgvmfwaiw sdlitvfsyl dsitlwhyng seagaavvks
      841 vtmgsllfai iaamvawali rnlpgllevl vlsrlnmrqg asyaittiln yviiavgamt
      901 vfgslgvswd klqwlaaals vglgfglqei fgnfvsglii lferpvrigd tvtigtysgt
      961 vskiriratt itdfdrkevi ipnkafvter linwslsdtt trlvirlgva ygsdlekvkr
     1021 vllqaamehp kvmhdpepav ffttfgastl dhelrlyvre lrdrshtvde lnraidrlcr
     1081 endiniafnq levhlhnakg devtevkrdl nggdlaptas