Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]

sulfur transfer protein (from cys to ThiS and from IscS to U8-tRNA)


LOCUS       NP_459420                482 aa            linear   CON 08-SEP-2022
            [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2].
ACCESSION   NP_459420
VERSION     NP_459420.1
DBLINK      BioProject: PRJNA57799
            BioSample: SAMN02604315
DBSOURCE    REFSEQ: accession NC_003197.2
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 482)
  AUTHORS   McClelland,M., Sanderson,K.E., Spieth,J., Clifton,S.W.,
            Latreille,P., Courtney,L., Porwollik,S., Ali,J., Dante,M., Du,F.,
            Hou,S., Layman,D., Leonard,S., Nguyen,C., Scott,K., Holmes,A.,
            Grewal,N., Mulvaney,E., Ryan,E., Sun,H., Florea,L., Miller,W.,
            Stoneking,T., Nhan,M., Waterston,R. and Wilson,R.K.
  TITLE     Complete genome sequence of Salmonella enterica serovar Typhimurium
            LT2
  JOURNAL   Nature 413 (6858), 852-856 (2001)
   PUBMED   11677609
REFERENCE   2  (residues 1 to 482)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (residues 1 to 482)
  AUTHORS   McClelland,M., Jain,A., Saraogi,P., Mendelson,R., Westerman,R.,
            SanMiguel,P. and Csonka,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2016) Department of Microbiology and Molecular
            Genetics, University of California, Irvine, CA 92697, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (residues 1 to 482)
  CONSRTM   The Salmonella typhimurium Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2001) Genome Sequencing Center, Department of
            Genetics, Washington University School of Medicine, 4444 Forest
            Park Boulevard, St. Louis, MO 63108, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAL19379.
            Supported by NIH grant 5U 01 AI43283
            
            Coding sequences below are predicted from manually evaluated
            computer analysis, using similarity information and the programs;
            GLIMMER; http://www.tigr.org/softlab/glimmer/glimmer.html and
            GeneMark; http://opal.biology.gatech.edu/GeneMark/
            
            EC numbers were kindly provided by Junko Yabuzaki and the Kyoto
            Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/,
            and Pedro Romero and Peter Karp at EcoCyc;
            http://ecocyc.PangeaSystems.com/ecocyc/
            
            The analyses of ribosome binding sites and promoter binding sites
            were kindly provided by Heladia Salgado, Julio Collado-Vides and
            ReguonDB;
            http://kinich.cifn.unam.mx:8850/db/regulondb_intro.frameset
            
            This sequence was finished as follows unless otherwise noted: all
            regions were double stranded, sequenced with an alternate
            chemistries or covered by high quality data (i.e., phred quality >=
            30); an attempt was made to resolve all sequencing problems, such
            as compressions and repeats; all regions were covered by sequence
            from more than one m13 subclone.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium str. LT2"
                     /strain="LT2"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /culture_collection="ATCC:700720"
                     /culture_collection="SGSC:1412"
                     /type_material="type strain of Salmonella enterica"
                     /db_xref="taxon:99287"
                     /focus
     Protein         1..482
                     /product="sulfur transfer protein (from cys to ThiS and
                     from IscS to U8-tRNA)"
                     /calculated_mol_wt=54700
     Region          3..379
                     /region_name="ThiI"
                     /note="Adenylyl- and sulfurtransferase ThiI (thiamine and
                     tRNA 4-thiouridine biosynthesis) [Coenzyme transport and
                     metabolism, Translation, ribosomal structure and
                     biogenesis]; COG0301"
                     /db_xref="CDD:440070"
     Region          382..482
                     /region_name="ThiI_C_thiazole"
                     /note="thiazole biosynthesis domain; TIGR04271"
                     /db_xref="CDD:275094"
     Site            456
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238089"
     CDS             1..482
                     /gene="thiI"
                     /locus_tag="STM0425"
                     /coded_by="NC_003197.2:477869..479317"
                     /note="thiamine biosynthesis protein thiI.
                     (SW:THII_SALTY)"
                     /transl_table=11
                     /db_xref="GeneID:1251944"
CONTIG      join(WP_000668708.1:1..482)
ORIGIN      
        1 mkfiiklfpe itiksqsvrl rfikiltgni rnvlkhydet lavvrhwdni evrakdenqr
       61 lvirdaltri pgihhileve dvpftdmhdi fekalaqyre qlegktfcvr vkrrgkhefs
      121 sieveryvgg glnqhiesar vkltnpdvtv hleveddrll likgryegig gfpigtqedv
      181 lslisggfds gvssymlmrr gcrvhycffn lggaaheigv rqvahylwnr fgsshrvrfv
      241 ainfepvvge ilekvddgqm gvvlkrmmvr aaskvaeryg vqalvtgeal gqvssqtltn
      301 lrlidnvsdt lilrplisyd kehiinlarq igtedfartm peycgvisks ptvkaikaki
      361 eaeeenfdfs ildkvveean nvdireiaqq tqqevvevet vsgfgpndvi ldirsvdeqd
      421 dkplkvegvd vvslpfykls tkfgdldqsk twllwcergv msrlqalylr eqgfanvkvy
      481 rp