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RNA helicase [Escherichia coli O157:H7 str. Sakai].


LOCUS       NP_313290               2113 aa            linear   CON 12-FEB-2021
ACCESSION   NP_313290
VERSION     NP_313290.1
DBLINK      BioProject: PRJNA226
            BioSample: SAMN01911278
DBSOURCE    REFSEQ: accession NC_002695.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7 str. Sakai
  ORGANISM  Escherichia coli O157:H7 str. Sakai
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K.,
            Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T.,
            Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C.,
            Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and
            Shinagawa,H.
  TITLE     Complete genome sequence of enterohemorrhagic Escherichia coli
            O157:H7 and genomic comparison with a laboratory strain K-12
  JOURNAL   DNA Res. 8 (1), 11-22 (2001)
   PUBMED   11258796
  REMARK    Erratum:[DNA Res 2001 Apr 27;8(2):96]
REFERENCE   2
  AUTHORS   Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S.,
            Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I.,
            Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T.,
            Honda,T., Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT1-Sakai carrying the
            Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli
            O157:H7 strain derived from the Sakai outbreak
  JOURNAL   Gene 258 (1-2), 127-139 (2000)
   PUBMED   11111050
REFERENCE   3
  AUTHORS   Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M.,
            Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and
            Hayashi,T.
  TITLE     Comparative analysis of the whole set of rRNA operons between an
            enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an
            Escherichia coli K-12 strain MG1655
  JOURNAL   Syst. Appl. Microbiol. 23 (3), 315-324 (2000)
   PUBMED   11108008
REFERENCE   4
  AUTHORS   Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S.,
            Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T.,
            Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T.,
            Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT2-Sakai carrying the
            verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7
            derived from the Sakai outbreak
  JOURNAL   Genes Genet. Syst. 74 (5), 227-239 (1999)
   PUBMED   10734605
REFERENCE   5  (residues 1 to 2113)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2018) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   6  (residues 1 to 2113)
  AUTHORS   Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University;
            31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to BAB38686.
            On May 10, 2018 this sequence version replased gi: 47118301
            
            This update is obtained as follows: the Sakai genome was
            resequenced by illumina MiSeq and PacBio RS II sequencers and 78
            sites of single base error, 11 sites of insertion (10 one-base
            insertions and a 141-base insertion) and 16 sites of deletion (14
            one-base deletions, a three-base deletion and a six-base deletion)
            were corrected. Annotation was performed using DFAST
            auto-annotation system with manual curation.
            
            This work was done in collaboration with Tetsuya Hayashi, Makoto
            Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino,
            Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda,
            Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata
            (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe,
            Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine
            Science and Technology Center), Naotake Ogasawara (Nara Institute
            of Science and Technology), Satoru Kuhara (Kuyshu University), and
            supported by the Research for the Future Program of the Japan
            Society for the Promotion of Science.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli O157:H7 str. Sakai"
                     /strain="Sakai"
                     /sub_strain="RIMD 0509952"
                     /serovar="O157:H7"
                     /db_xref="taxon:386585"
     Protein         1..2113
                     /product="RNA helicase"
                     /calculated_mol_wt=238103
     Region          81..288
                     /region_name="DEXHc_Hrq1-like"
                     /note="DEAH-box helicase domain of Hrq1 and similar
                     proteins; cd17923"
                     /db_xref="CDD:350681"
     Site            order(105..112,148,234..235)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350681"
     Region          <998..>1126
                     /region_name="YprA"
                     /note="ATP-dependent helicase YprA, contains C-terminal
                     metal-binding DUF1998 domain [Replication, recombination
                     and repair]; COG1205"
                     /db_xref="CDD:440818"
     Region          1548..1638
                     /region_name="MZB"
                     /note="MrfA Zn-binding domain; pfam09369"
                     /db_xref="CDD:462776"
     CDS             1..2113
                     /locus_tag="ECs_5263"
                     /coded_by="NC_002695.2:5381703..5388044"
                     /transl_table=11
                     /db_xref="GeneID:913721"
CONTIG      join(WP_000937974.1:1..2113)
ORIGIN      
        1 mlpsvvsrqv adsvaaflra afplnsplfn genndnvsml eqflaqpetl lkgpylsaql
       61 pfrksdlpln ffpnltlpfp phahqarafq rlgsdapqpt lvatgtgsgk tecfmfplln
      121 hcagasqagv kaiiiypmna latdqasrfa ktiasdpqlh gkvtvglfvg dseiepskkm
      181 saekvitckh tlresppdil ltnykmldyl lmrpgdqplw rynqpgslry lvvdelhtfd
      241 gaqgsdlacl vrrlkhhigv dnqrfacvgt satvgdelgq lldyaktifd qpftddavir
      301 edrytaaeyl qnyaiaysqy pgpevasald pqsyatpvay lngqiplwfp dsdlqlpddl
      361 esddgrekri algsllrrhs vmhvllhdlq ggilserecl enlqvmlaes adharrvlqs
      421 ilaliaqarl evpetehdrq krlqakkarp vlpfvqlrsq lwlremrrlv asvaktprlv
      481 faddvavedr ehylpvihcr dchatgwasl rhgqsvqlet dldgiyrqff ekgrsivlaf
      541 pdnnekavsg ahqklcptcl klnrqssvqc ghcghtellq vlipdmlker kdelefanec
      601 pycnskqgis ilgsraasls avmihqlygs qfnehkklit fsdsvqdaah rasfftartw
      661 plmirgqias vlseketlpl dafaarvcqq trerspddah faanviapnm qwlndyqtlk
      721 negklaensd lpelvslrld wevyseftlr arigrtleat gyavagvdkd rfnpaitalh
      781 qqlceelgde msgvsveqva hlalgviwyq rqagavmhpa fesyrrdgtt fmmtqkerts
      841 rympsigpkt rkpayasfek ingfhrliga ksnpswyqhw inrtlsngnn lfissvaetv
      901 lrrlftalki agvvkdfdtk greawglvps alvvsrdvvq lncsccrevv rapadqgwqw
      961 rnapclslrc egryqevdnt atwrwenmdi arvqgaehtg llsredrevt eksfyrgnqp
     1021 wninllsatp tlemgidvgd lstvllcsvp paqanylqri gragrkdgna lnitvaegnp
     1081 hdqfffeepl emmqgqvqap gvflnatail erqlaafcmd nwvktgvpes aicknvkqml
     1141 delefgrksg fpynllryie qhhaeiaaqf taifpdlaae trqqllsylq gapgqrslvq
     1201 rieealkllv edrkslrsri dklkrsidkl enaprdqnfd sdmreltser qalmalvnqi
     1261 nnkqtlnflt degllpnyaf peagitlrsv lwrrkeggea reyqnttfey erpastalae
     1321 laplnnfyag ghkveieqid lkvsepenwr icshcnysen idqtgdqhky cpkcgtpgwa
     1381 dagqkttllk lrqvyarasa rdsqisdesd srepaffqrq llvsfekedv saayaieege
     1441 vpfgfeflsk vtlrdinfgk maddanelmi ageakkrtgf kvclgcgmvq rprdheprhd
     1501 lsckyrtepe kakfedylyl yrqlesealr illpvssysn drvveaslga aiqlglkhyf
     1561 kgnvdhlkgv vyrepenege swrqylviyd tvpggtgslk elmrtpdnll kllelayqai
     1621 vecscnndth kdgcyrcvya yrdrgrmkyv srdqarllla kilqardsir vidsiknisl
     1681 eammgselek rfisclqenk nlivsrsyah qgagwiintr sepmmswhlk aqvdlgakea
     1741 vgipcrpdyv lyplmqsdai kpvaifldgf afhknsvaed vqkrqairds gnfwvwtvtw
     1801 adlkepglkh vqdvlglghn pdmkqpkfyn lfhdtnfaal easfrernsf allldyladp
     1861 ggrtqlwqrm aaahawvwld vkksqdagtk qkyayemqen apawrlaell pdepfvfggl
     1921 ldscnssqqf ielasvlpqq aikpttsvaq mrswlrlhic fddrytqddg yeaglngfwr
     1981 lvnllqflpd mtftsrkavh lpqesvdtkv satpeavpet deswaeiief gllsaeeial
     2041 lqshslsapt lgyelqndeg eiiaeadlaw psqkwaliid nqefiplftt rgwhvafgpi
     2101 dentlqhlsg gdk