bacterial alpha2-macroglobulin colonization factor ECAM

LOCUS       NP_311413               1653 aa            linear   CON 12-FEB-2021
            [Escherichia coli O157:H7 str. Sakai].
ACCESSION   NP_311413
VERSION     NP_311413.1
DBLINK      BioProject: PRJNA226
            BioSample: SAMN01911278
DBSOURCE    REFSEQ: accession NC_002695.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7 str. Sakai
  ORGANISM  Escherichia coli O157:H7 str. Sakai
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K.,
            Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T.,
            Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C.,
            Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and
            Shinagawa,H.
  TITLE     Complete genome sequence of enterohemorrhagic Escherichia coli
            O157:H7 and genomic comparison with a laboratory strain K-12
  JOURNAL   DNA Res. 8 (1), 11-22 (2001)
   PUBMED   11258796
  REMARK    Erratum:[DNA Res 2001 Apr 27;8(2):96]
REFERENCE   2
  AUTHORS   Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S.,
            Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I.,
            Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T.,
            Honda,T., Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT1-Sakai carrying the
            Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli
            O157:H7 strain derived from the Sakai outbreak
  JOURNAL   Gene 258 (1-2), 127-139 (2000)
   PUBMED   11111050
REFERENCE   3
  AUTHORS   Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M.,
            Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and
            Hayashi,T.
  TITLE     Comparative analysis of the whole set of rRNA operons between an
            enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an
            Escherichia coli K-12 strain MG1655
  JOURNAL   Syst. Appl. Microbiol. 23 (3), 315-324 (2000)
   PUBMED   11108008
REFERENCE   4
  AUTHORS   Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S.,
            Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T.,
            Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T.,
            Sasakawa,C. and Shinagawa,H.
  TITLE     Complete nucleotide sequence of the prophage VT2-Sakai carrying the
            verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7
            derived from the Sakai outbreak
  JOURNAL   Genes Genet. Syst. 74 (5), 227-239 (1999)
   PUBMED   10734605
REFERENCE   5  (residues 1 to 1653)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2018) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   6  (residues 1 to 1653)
  AUTHORS   Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University;
            31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to BAB36809.
            On May 10, 2018 this sequence version replased gi: 47118301
            
            This update is obtained as follows: the Sakai genome was
            resequenced by illumina MiSeq and PacBio RS II sequencers and 78
            sites of single base error, 11 sites of insertion (10 one-base
            insertions and a 141-base insertion) and 16 sites of deletion (14
            one-base deletions, a three-base deletion and a six-base deletion)
            were corrected. Annotation was performed using DFAST
            auto-annotation system with manual curation.
            
            This work was done in collaboration with Tetsuya Hayashi, Makoto
            Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino,
            Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda,
            Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata
            (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe,
            Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine
            Science and Technology Center), Naotake Ogasawara (Nara Institute
            of Science and Technology), Satoru Kuhara (Kuyshu University), and
            supported by the Research for the Future Program of the Japan
            Society for the Promotion of Science.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Escherichia coli O157:H7 str. Sakai"
                     /strain="Sakai"
                     /sub_strain="RIMD 0509952"
                     /serovar="O157:H7"
                     /db_xref="taxon:386585"
     Protein         1..1653
                     /product="bacterial alpha2-macroglobulin colonization
                     factor ECAM"
                     /calculated_mol_wt=181286
     Region          32..1652
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     CDS             1..1653
                     /gene="yfhM"
                     /locus_tag="ECs_3386"
                     /coded_by="complement(NC_002695.2:3368433..3373394)"
                     /transl_table=11
                     /db_xref="GeneID:912952"
CONTIG      join(WP_000736260.1:1..1653)
ORIGIN      
        1 mkklrvaacm lmlalagcdn ndnaptavkk dapsevtkaa ssenassakl saperqklaq
       61 qsagkaltll dlsevqldga atlvltfsip ldpdqdfsrv ihvvdkksgk vdgawelsdn
      121 lkelrlrhle pkrdlivtig kevkalnnat fskdyektit trdiqpsvgf asrgsllpgk
      181 vveglpvmal nvnnvdvnff rvkpeslpaf isqweyrnsl anwqsdkllq madlvytgrf
      241 dlnparntre klllplgdik plqqagvyla vmnqagrydy snpatlftls digvsahryh
      301 nrldiftqsl engaaqqgie vsllnekgqt ltqatsdaqg hvqlendkna alllarkdgq
      361 ttlldlklpa ldlaefniag apgyskqffm fgprdlyrpg etvilngllr dadgkalpnq
      421 pikldvikpd gqvlrsvvsq penglyhftw pldsnaatgm whirantgdn qyrmwdfhve
      481 dfmpermaln ltgektpltp ndevkfsvvg yylygapang ntlqgqlflr plreavsalp
      541 gfefgdiaae nlsrtldevq ltlddkgrge vstesqwket hsplqvifqg sllesggrpv
      601 trraeqaiwp adalpgirpq fasksvydyr tdstvkqpiv degsnaafdi vysdaqgvkk
      661 avsglqvrli rerrdyywnw sedegwqsqf dqkdlieneq tldlkadetg kvsfpvewga
      721 yrlevkapne avssvrfwag yswqdnsdgs gavrpdrvtl kldkagyrpg dtiklhiaap
      781 tagkgyamve ssegplwwqe idvpaqgldl tipvdktwnr hdlylstlvv rpgdksrsat
      841 pkravgvlhl plgdenrrld laletpakmr pnqpltvkik astkngekpk qvnvlvsavd
      901 sgvlnitdyv tpdpwqaffg qkrygadiyd iygqviegqg rlaalrfggd gdelkrggkp
      961 pvnhvnivaq qalpvtlneq gegsvtlpig dfngelrvma qawtadefgs neskvivaap
     1021 viaelnmprf masgdnsrlt lditnltdkp qklnvaltas gllelvsdsp aavelapgvr
     1081 ttlfipvral pgygdgeiqa tisglalpge tvadqhkqwk igvrpafpaq tvnygtalqp
     1141 getwaipadg lqnfspvtle gqlllsgkpp lniaryikel kaypygcleq tasglfpsly
     1201 tnaaqlqalg ikgdsdekrr asvdigisrl lqmqrdnggf alwdkngdee ywltayvmdf
     1261 lvrageqgys vptdainrgn erllrylqdp gmmsipyadn lkaskfavqs yaalvlarqq
     1321 kaplgalrei wehradaasg lpllqlgval ktmgdatrde eaialalktp rnsderiwlg
     1381 dygsplrdna lmlslleenk llpdeqysll ntlsqqafge rwlstqesna lflaartiqd
     1441 lpgkwqaqts fsaepltgek aqnsnlnsdq latlqvtnsg eqplwlrvda sgypqsaplp
     1501 annvlqierh ilgtdgksks ldslrsgdlv lvwlqvkasn svpdalvvdl lpaglelenq
     1561 nlangsasle qsggevqnll nqmqqasikh iefrddrfva avavdeyqpv tlvylaravt
     1621 pgtyqvpqpm vesmyvpqwr atgaaedlli vrp