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LOCUS NP_309287 1320 aa linear CON 12-FEB-2021 dehydrogenase [Escherichia coli O157:H7 str. Sakai]. ACCESSION NP_309287 VERSION NP_309287.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 1320) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 1320) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB34683. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1320 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..1320 /product="bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase" /calculated_mol_wt=143699 Region 1..1320 /region_name="putA" /note="trifunctional transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase; Reviewed; PRK11809" /db_xref="CDD:236989" CDS 1..1320 /gene="putA" /locus_tag="ECs_1260" /coded_by="complement(NC_002695.2:1315882..1319844)" /transl_table=11 /db_xref="GeneID:912875" CONTIG join(WP_001356407.1:1..1320) ORIGIN 1 mgtttmgvkl ddatreriks aatridrtph wlikqaifsy leqlensdtl pelpallsga 61 anesdeaptp aeephqpfld faeqilpqsv sraaitaayr rpeteavsml leqarlpqpv 121 aeqahklayq ladklrnqkn asgragmvqg llqefslssq egvalmclae allripdkat 181 rdalirdkis ngnwqshigr spslfvnaat wgllftgklv sthneaslsr slnriigksg 241 eplirkgvdm amrlmgeqfv tgetiaeala narkleekgf rysydmlgea altaadaqay 301 mvsyqqaiha igkasngrgi yegpgisikl salhprysra qydrvmeely prlksltlla 361 rqydiginid aeeadrleis ldlleklcfe pelagwngig fviqayqkrc plvidylidl 421 atrsrrrlmi rlvkgaywds eikraqmdgl egypvytrkv ytdvsylaca kkllavpnli 481 ypqfathnah tlaaiyqlag qnyypgqyef qclhgmgepl yeqvtgkvad gklnrpcriy 541 apvgthetll aylvrrllen gantsfvnri adtslpldel vadpvtavek laqqegqtgl 601 phpkiplprd lyghgrdnsa gldlanehrl aslssallns alqkwqalpm leqpvaagem 661 spvinpaepk divgyvreat preveqales avnnapiwfa tppaeraail hraavlmesq 721 mqqligilvr eagktfsnai aevreavdfl hyyagqvrdd fanethrplg pvvcispwnf 781 plaiftgqia aalaagnsvl akpaeqtpli aaqgiaille agvppgvvql qpgrgetvga 841 qltgddrvrg vmftgsteva tllqrniasr ldaqgrpipl iaetggmnam ivdssalteq 901 vvidvlasaf dsagqrcsal rvlclqdeia dhtlkmlrga maecrmgnpg rlttdigpvi 961 dseakanier hiqtmrskgr pvfqavrens edarewqsgt fvaptlield dfaelqkevf 1021 gpvlhvvryn rnqlpelieq inasgygltl gvhtrideti aqvtgsahvg nlyvnrnmvg 1081 avvgvqpfgg eglsgtgpka ggplylyrll anrpesalav tlarqdaeyp vdaqlkaalt 1141 qplnalrewa anrpelqalc tqygelaqag tqrllpgptg erntwtllpr ervlciadde 1201 qdaltqlaav lavgsqvlwp ddalhrqlvk alpstvseri qlakaenita qpfdavifhg 1261 dsdqlralce avaardgaiv svqgfarges nillerlyie rslsvntaaa ggnaslmtig