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LOCUS NP_308149 865 aa linear CON 12-FEB-2021 [Escherichia coli O157:H7 str. Sakai]. ACCESSION NP_308149 VERSION NP_308149.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 865) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 865) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB33545. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..865 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..865 /product="bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase" /calculated_mol_wt=93353 Region 1..864 /region_name="AcnB" /note="Aconitase B [Energy production and conversion]; COG1049" /db_xref="CDD:440670" CDS 1..865 /gene="acnB" /locus_tag="ECs_0122" /coded_by="NC_002695.2:135988..138585" /transl_table=11 /db_xref="GeneID:913670" CONTIG join(WP_001303793.1:1..865) ORIGIN 1 mleeyrkhva eraaegiapk pldanqmaal vellknppag eeeflldllt nrvppgvdea 61 ayvkagflaa vakgeakspl ltpekaiell gtmqggynih plidalddak lapiaakals 121 htllmfdnfy dveekakagn eyakqvmqsw adaewflnrp alaekltvtv fkvtgetntd 181 dlspapdaws rpdiplhala mlknaregie pdqpgvvgpi kqiealqqkg fplayvgdvv 241 gtgssrksat nsvlwfmgdd iphvpnkrgg glclggkiap iffntmedag alpievdvsn 301 lnmgdvidvy pykgevrnhe tgellatfel ktdvlidevr aggripliig rglttkarea 361 lglphsdvfr qakdvaesdr gfslaqkmvg racgvkgirp gaycepkmts vgsqdttgpm 421 trdelkdlac lgfsadlvmq sfchtaaypk pvdvnthhtl pdfimnrggv slrpgdgvih 481 swlnrmllpd tvgtggdsht rfpigisfpa gsglvafaaa tgvmpldmpe svlvrfkgkm 541 qpgitlrdlv haiplyaikq glltvekkgk knifsgrile ieglpdlkve qafeltdasa 601 ersaagctik lnkepiieyl nsnivllkwm iaegygdrrt lerriqgmek wlanpellea 661 dadaeyaavi didladikep ilcapndpdd arplsavqge kidevfigsc mtnighfraa 721 gklldahkgq lptrlwvapp trmdaaqlte egyysvfgks garieipgcs lcmgnqarva 781 dgatvvstst rnfpnrlgtg anvflasael aavaaligkl ptpeeyqtyv aqvdktavdt 841 yrylnfnqls qytekadgvi fqtav