Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]
LOCUS NP_308056 938 aa linear CON 12-FEB-2021 ACCESSION NP_308056 VERSION NP_308056.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 938) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 938) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB33452. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..938 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..938 /product="isoleucyl-tRNA synthetase" /calculated_mol_wt=104194 Region 4..930 /region_name="IleS" /note="Isoleucyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; COG0060" /db_xref="CDD:439830" CDS 1..938 /gene="ileS" /locus_tag="ECs_0029" /coded_by="NC_002695.2:26803..29619" /transl_table=11 /db_xref="GeneID:913425" CONTIG join(WP_001286832.1:1..938) ORIGIN 1 msdykstlnl petgfpmrgd lakrepgmla rwtdddlygi iraakkgkkt filhdgppya 61 ngsihighsv nkilkdiivk skglsgydsp yvpgwdchgl pielkveqey gkpdekftaa 121 efrakcreya atqvdgqrkd firlgvlgdw shpyltmdfk teaniiralg kiignghlhk 181 gakpvhwcvd crsalaeaev eyydktspsi dvafqavdqd alkakfgvsn vngpislviw 241 tttpwtlpan raisiapdfd yalvqidgqa vilakdlves vmqrigvtdy tilgtvkgae 301 lellrfahpf mgfdvpailg dhvtldagtg avhtapghgp ddyvigqkyg letanpvgpd 361 gtylpgtypt ldgvnvfkan divvallqek gallhvekmq hsypccwrhk tpiifratpq 421 wfvsmdqkgl raqslkeikg vqwipdwgqa riesmvanrp dwcisrqrtw gvpmslfvhk 481 dteelhprtl elmeevakrv evdgiqawwd ldakeilgde adqyvkvpdt ldvwfdsgst 541 hssvvdvrpe faghaadmyl egsdqhrgwf msslmistam kgkapyrqvl thgftvdgqg 601 rkmsksignt vspqdvmnkl gadilrlwva stdytgemav sdeilkraad syrrirntar 661 fllanlngfd pakdmvkpee mvvldrwavg cakaaqedil kayeaydfhe vvqrlmrfcs 721 vemgsfyldi ikdrqytaka dsvarrscqt alyhiaealv rwmapilsft adevwgylpg 781 erekyvftge wyeglfglad seamndafwd ellkvrgevn kvieqaradk kvggsleaav 841 tlytepelaa kltalgdelr fvlltsgatv adyndapada qqsevlkglk valskaegek 901 cprcwhytqd vgkvaehaei cgrcvsnvag dgekrkfa