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isoleucine--tRNA ligase [Escherichia coli].


LOCUS       WP_001286832             938 aa            linear   BCT 23-MAY-2024
ACCESSION   WP_001286832
VERSION     WP_001286832.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 938)
  AUTHORS   Fukunaga,R. and Yokoyama,S.
  TITLE     Crystallization and preliminary X-ray crystallographic study of the
            editing domain of Thermus thermophilus isoleucyl-tRNA synthetase
            complexed with pre- and post-transfer editing-substrate analogues
  JOURNAL   Acta Crystallogr D Biol Crystallogr 60 (Pt 10), 1900-1902 (2004)
   PUBMED   15388946
REFERENCE   2  (residues 1 to 938)
  AUTHORS   Yanagisawa,T. and Kawakami,M.
  TITLE     How does Pseudomonas fluorescens avoid suicide from its antibiotic
            pseudomonic acid?: Evidence for two evolutionarily distinct
            isoleucyl-tRNA synthetases conferring self-defense
  JOURNAL   J Biol Chem 278 (28), 25887-25894 (2003)
   PUBMED   12672810
REFERENCE   3  (residues 1 to 938)
  AUTHORS   Nakama,T., Nureki,O. and Yokoyama,S.
  TITLE     Structural basis for the recognition of isoleucyl-adenylate and an
            antibiotic, mupirocin, by isoleucyl-tRNA synthetase
  JOURNAL   J Biol Chem 276 (50), 47387-47393 (2001)
   PUBMED   11584022
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00392.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..938
                     /gene="ileS"
     Protein         1..938
                     /product="isoleucine--tRNA ligase"
                     /EC_number="6.1.1.5"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /calculated_mol_wt=104194
     Region          4..930
                     /region_name="IleS"
                     /note="Isoleucyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0060"
                     /db_xref="CDD:439830"
ORIGIN      
        1 msdykstlnl petgfpmrgd lakrepgmla rwtdddlygi iraakkgkkt filhdgppya
       61 ngsihighsv nkilkdiivk skglsgydsp yvpgwdchgl pielkveqey gkpdekftaa
      121 efrakcreya atqvdgqrkd firlgvlgdw shpyltmdfk teaniiralg kiignghlhk
      181 gakpvhwcvd crsalaeaev eyydktspsi dvafqavdqd alkakfgvsn vngpislviw
      241 tttpwtlpan raisiapdfd yalvqidgqa vilakdlves vmqrigvtdy tilgtvkgae
      301 lellrfahpf mgfdvpailg dhvtldagtg avhtapghgp ddyvigqkyg letanpvgpd
      361 gtylpgtypt ldgvnvfkan divvallqek gallhvekmq hsypccwrhk tpiifratpq
      421 wfvsmdqkgl raqslkeikg vqwipdwgqa riesmvanrp dwcisrqrtw gvpmslfvhk
      481 dteelhprtl elmeevakrv evdgiqawwd ldakeilgde adqyvkvpdt ldvwfdsgst
      541 hssvvdvrpe faghaadmyl egsdqhrgwf msslmistam kgkapyrqvl thgftvdgqg
      601 rkmsksignt vspqdvmnkl gadilrlwva stdytgemav sdeilkraad syrrirntar
      661 fllanlngfd pakdmvkpee mvvldrwavg cakaaqedil kayeaydfhe vvqrlmrfcs
      721 vemgsfyldi ikdrqytaka dsvarrscqt alyhiaealv rwmapilsft adevwgylpg
      781 erekyvftge wyeglfglad seamndafwd ellkvrgevn kvieqaradk kvggsleaav
      841 tlytepelaa kltalgdelr fvlltsgatv adyndapada qqsevlkglk valskaegek
      901 cprcwhytqd vgkvaehaei cgrcvsnvag dgekrkfa