MULTISPECIES: tRNA 2-thiouridine(34) synthase MnmA [Vibrio].

LOCUS       WP_017043901             373 aa            linear   BCT 27-DEC-2023
ACCESSION   WP_017043901
VERSION     WP_017043901.1
KEYWORDS    RefSeq.
SOURCE      Vibrio
  ORGANISM  Vibrio
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Vibrionales; Vibrionaceae.
REFERENCE   1  (residues 1 to 373)
  AUTHORS   Numata,T., Ikeuchi,Y., Fukai,S., Suzuki,T. and Nureki,O.
  TITLE     Snapshots of tRNA sulphuration via an adenylated intermediate
  JOURNAL   Nature 442 (7101), 419-424 (2006)
   PUBMED   16871210
REFERENCE   2  (residues 1 to 373)
  AUTHORS   Kambampati,R. and Lauhon,C.T.
  TITLE     MnmA and IscS are required for in vitro 2-thiouridine biosynthesis
            in Escherichia coli
  JOURNAL   Biochemistry 42 (4), 1109-1117 (2003)
   PUBMED   12549933
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00420.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Vibrio"
                     /db_xref="taxon:662"
     gene            1..373
                     /gene="mnmA"
                     /gene_synonym="trmU"
     Protein         1..373
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /EC_number="2.8.1.13"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /calculated_mol_wt=41661
     Region          11..373
                     /region_name="MnmA"
                     /note="tRNA U34 2-thiouridine synthase MnmA/TrmU, contains
                     the PP-loop ATPase domain [Translation, ribosomal
                     structure and biogenesis]; COG0482"
                     /db_xref="CDD:440250"
ORIGIN      
        1 msdnrsansq kkvivgmsgg vdssvsayll keqgyqvegl fmknweeddn eeyctaaedl
       61 adaqavcdkl diplhtinfa aeywdnvfey fleeykagrt pnpdilcnke ikfkaflefa
      121 devldadyia mghyvrrtfp egndkpkmlr gldnnkdqsy flytlsheqv arslfpvgdl
      181 ekpevrriae eqglvtakkk dstgicfige rkftdflsry lpaqpgkiet peglvigehq
      241 glmyhtlgqr kglhiggqkg gggnedpwyv adkdlkrnvl iavqgadhpl lkshgliasq
      301 lhwvdrtpiq atlsctvktr yrqtdiacti ipiddenikv ifdepqvavt pgqsavfyqg
      361 dvclgggiie qri