MULTISPECIES: tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase MiaE

LOCUS       WP_017004115             259 aa            linear   BCT 09-JAN-2025
            [Enterovibrio].
ACCESSION   WP_017004115
VERSION     WP_017004115.1
KEYWORDS    RefSeq.
SOURCE      Enterovibrio
  ORGANISM  Enterovibrio
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Vibrionales; Vibrionaceae.
REFERENCE   1  (residues 1 to 259)
  AUTHORS   Mathevon,C., Pierrel,F., Oddou,J.L., Garcia-Serres,R., Blondin,G.,
            Latour,J.M., Menage,S., Gambarelli,S., Fontecave,M. and Atta,M.
  TITLE     tRNA-modifying MiaE protein from Salmonella typhimurium is a
            nonheme diiron monooxygenase
  JOURNAL   Proc Natl Acad Sci U S A 104 (33), 13295-13300 (2007)
   PUBMED   17679698
REFERENCE   2  (residues 1 to 259)
  AUTHORS   Persson,B.C. and Bjork,G.R.
  TITLE     Isolation of the gene (miaE) encoding the hydroxylase involved in
            the synthesis of 2-methylthio-cis-ribozeatin in tRNA of Salmonella
            typhimurium and characterization of mutants
  JOURNAL   J Bacteriol 175 (24), 7776-7785 (1993)
   PUBMED   8253666
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF047790.1
            Evidence Source    :: NCBIFAM
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Enterovibrio"
                     /db_xref="taxon:188143"
     gene            1..259
                     /gene="miaE"
     Protein         1..259
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /EC_number="1.14.99.69"
                     /GO_function="GO:0004497 - monooxygenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence
                     IEA]"
                     /calculated_mol_wt=29153
     Region          10..252
                     /region_name="MiaE"
                     /note="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase MiaE
                     (synthesis of 2-methylthio-cis-ribozeatin) [Translation,
                     ribosomal structure and biogenesis]; COG4445"
                     /db_xref="CDD:443544"
ORIGIN      
        1 mslsqelekl lepirgflhc etpqawidea ikpenetill rdhancelka sqtamwlirk
       61 yaidessgal llewakpyed fvylckkdgi sqskknglsa pltpkagfah sqdlidkmvr
      121 likeefhhfe qvveimekrd maysslpagg yarglmksar thepatlidk liigayiear
      181 scerfakvap yldeelrkfy isllrsearh yqdylklaea iaggdisdri kiigekeaal
      241 iqapdntfrf hsgvpalaa