tRNA 2-thiouridine(34) synthase MnmA [Salmonella enterica].

LOCUS       WP_014343832             383 aa            linear   BCT 27-DEC-2023
ACCESSION   WP_014343832
VERSION     WP_014343832.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 383)
  AUTHORS   Numata,T., Ikeuchi,Y., Fukai,S., Suzuki,T. and Nureki,O.
  TITLE     Snapshots of tRNA sulphuration via an adenylated intermediate
  JOURNAL   Nature 442 (7101), 419-424 (2006)
   PUBMED   16871210
REFERENCE   2  (residues 1 to 383)
  AUTHORS   Kambampati,R. and Lauhon,C.T.
  TITLE     MnmA and IscS are required for in vitro 2-thiouridine biosynthesis
            in Escherichia coli
  JOURNAL   Biochemistry 42 (4), 1109-1117 (2003)
   PUBMED   12549933
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00420.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Salmonella enterica"
                     /db_xref="taxon:28901"
     gene            1..383
                     /gene="mnmA"
                     /gene_synonym="trmU"
     Protein         1..383
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /EC_number="2.8.1.13"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /calculated_mol_wt=42388
     Region          21..380
                     /region_name="MnmA"
                     /note="tRNA U34 2-thiouridine synthase MnmA/TrmU, contains
                     the PP-loop ATPase domain [Translation, ribosomal
                     structure and biogenesis]; COG0482"
                     /db_xref="CDD:440250"
ORIGIN      
        1 mieyaalkfn vasipmsesp kkvivgmsgg vdssvsawll qqqgyqvegl fmknweeddg
       61 eeyctaaadl adaqavcdkl gielhtvnfa aeywdnvfel fleeykagrt pnpdilcnke
      121 ikfkaflefa aedlgadyia tghyvrradv ngksrllrgl dgnkdqsyfl ytlgheqiaq
      181 slfpvgelek pqvrkiaedl glvtakkkds tgicfigerk frdflgrylp aqpgkiitvd
      241 gdeigehqgl myhtlgqrkg lgiggtkdgs edpwyvvdkd vennvlivaq ghehprlmsv
      301 gliaqqlhwv drepftgtlr ctvktryrqt dipctinaln ddrievifde pvaavtpgqs
      361 avfysgevcl gggiieqrlp ltv