MULTISPECIES: hydroxyethylthiazole kinase [Klebsiella].

LOCUS       WP_014343334             257 aa            linear   BCT 11-MAR-2024
ACCESSION   WP_014343334
VERSION     WP_014343334.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella
  ORGANISM  Klebsiella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group.
REFERENCE   1  (residues 1 to 257)
  AUTHORS   Drebes,J., Kunz,M., Windshugel,B., Kikhney,A.G., Muller,I.B.,
            Eberle,R.J., Oberthur,D., Cang,H., Svergun,D.I., Perbandt,M.,
            Betzel,C. and Wrenger,C.
  TITLE     Structure of ThiM from Vitamin B1 biosynthetic pathway of
            Staphylococcus aureus - Insights into a novel pro-drug approach
            addressing MRSA infections
  JOURNAL   Sci Rep 6, 22871 (2016)
   PUBMED   26960569
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 257)
  AUTHORS   Campobasso,N., Mathews,I.I., Begley,T.P. and Ealick,S.E.
  TITLE     Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase
            from Bacillus subtilis at 1.5 A resolution
  JOURNAL   Biochemistry 39 (27), 7868-7877 (2000)
   PUBMED   10891066
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF006830.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09355
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Klebsiella"
                     /db_xref="taxon:570"
     gene            1..257
                     /gene="thiM"
     Protein         1..257
                     /product="hydroxyethylthiazole kinase"
                     /EC_number="2.7.1.50"
                     /GO_function="GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=26100
     Region          10..253
                     /region_name="PRK09355"
                     /note="hydroxyethylthiazole kinase; Validated"
                     /db_xref="CDD:236477"
     Site            order(29,31,37,41,47,49,69,71..72)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238575"
     Site            order(30..35,37..38,41,49..52,54..55,58..59,71..74,
                     107..108,193..196,241..242,244..246,248..249,252)
                     /site_type="other"
                     /note="multimerization interface [polypeptide binding]"
                     /db_xref="CDD:238575"
     Site            order(98,124,126,129,170,172,174,192,198,200)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238575"
ORIGIN      
        1 mpellnpapv ahlrhllrah splvhcmtnd vvqtftanvl lavgaspamv idpreaaqfa
       61 aiadallinv gtltedrava mraaveharq agrpwtldpv avgaltvrta fchellvlqp
      121 aairgnasei lalagmsagg rgvdttdtaa aalpaaqala rrlatvvavt gevdyvtdge
      181 rvlsvaggnp lmtrvvgtgc alsavvaasa alpgdrlenv aaacglmkqa geiaarqggp
      241 gsfipaflda lyqevqg