MULTISPECIES: tRNA uracil 4-sulfurtransferase ThiI [Vibrio].

LOCUS       WP_014231021             482 aa            linear   BCT 24-APR-2023
ACCESSION   WP_014231021
VERSION     WP_014231021.1
KEYWORDS    RefSeq.
SOURCE      Vibrio
  ORGANISM  Vibrio
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Vibrionales; Vibrionaceae.
REFERENCE   1  (residues 1 to 482)
  AUTHORS   Martinez-Gomez,N.C., Palmer,L.D., Vivas,E., Roach,P.L. and
            Downs,D.M.
  TITLE     The rhodanese domain of ThiI is both necessary and sufficient for
            synthesis of the thiazole moiety of thiamine in Salmonella enterica
  JOURNAL   J Bacteriol 193 (18), 4582-4587 (2011)
   PUBMED   21724998
REFERENCE   2  (residues 1 to 482)
  AUTHORS   You,D., Xu,T., Yao,F., Zhou,X. and Deng,Z.
  TITLE     Direct evidence that ThiI is an ATP pyrophosphatase for the
            adenylation of uridine in 4-thiouridine biosynthesis
  JOURNAL   Chembiochem 9 (12), 1879-1882 (2008)
   PUBMED   18604845
REFERENCE   3  (residues 1 to 482)
  AUTHORS   Mueller,E.G., Palenchar,P.M. and Buck,C.J.
  TITLE     The role of the cysteine residues of ThiI in the generation of
            4-thiouridine in tRNA
  JOURNAL   J Biol Chem 276 (36), 33588-33595 (2001)
   PUBMED   11443125
REFERENCE   4  (residues 1 to 482)
  AUTHORS   Webb,E., Claas,K. and Downs,D.M.
  TITLE     Characterization of thiI, a new gene involved in thiazole
            biosynthesis in Salmonella typhimurium
  JOURNAL   J Bacteriol 179 (13), 4399-4402 (1997)
   PUBMED   9209060
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00342.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Vibrio"
                     /db_xref="taxon:662"
     gene            1..482
                     /gene="thiI"
     Protein         1..482
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /EC_number="2.8.1.4"
                     /GO_function="GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence
                     IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=54438
     Region          3..381
                     /region_name="ThiI"
                     /note="Adenylyl- and sulfurtransferase ThiI (thiamine and
                     tRNA 4-thiouridine biosynthesis) [Coenzyme transport and
                     metabolism, Translation, ribosomal structure and
                     biogenesis]; COG0301"
                     /db_xref="CDD:440070"
     Region          382..482
                     /region_name="ThiI_C_thiazole"
                     /note="thiazole biosynthesis domain; TIGR04271"
                     /db_xref="CDD:275094"
     Site            456
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238089"
ORIGIN      
        1 mkfivkphpe ifvksesvrk rftkilecni rnivksrtes vavfnrrdhi evtsesneyy
       61 aevleiltht pgihhvlevk qsefkdlhdi feqvlelsrp lienktfvvr akrrgkhdft
      121 sieleryvgg glnqavesas vklrnpdvtv kvevsgdkln qvlarhkglg gfplgtqedv
      181 lslisggfds gvssylhikr gskvhycffn lggpaheigv kqvshylwnk ygssakvrfi
      241 sidfepvvae ilekvddgqm gvilkrmfmr aagmvaqkfk iealvtgeal gqvssqtltn
      301 lrhidnvtdt lilrplinwd kediinlare igtedfaktm peycgviskk ptvkavkakl
      361 eaeeanfdft ilenvvkear vmdirdiake sekaapeveq vqaveehaiv ldirspeeed
      421 dnpleidgvd ikhipfyklg tqfgdldqdk tyllycdrgv msrlqalylq eqgfnnvkvy
      481 rp