MULTISPECIES: tRNA (N6-isopentenyl

LOCUS       WP_013857327             474 aa            linear   BCT 29-JUN-2021
            adenosine(37)-C2)-methylthiotransferase MiaB [Vibrio].
ACCESSION   WP_013857327
VERSION     WP_013857327.1
KEYWORDS    RefSeq.
SOURCE      Vibrio
  ORGANISM  Vibrio
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Vibrionales; Vibrionaceae.
REFERENCE   1  (residues 1 to 474)
  AUTHORS   Pierrel,F., Hernandez,H.L., Johnson,M.K., Fontecave,M. and Atta,M.
  TITLE     MiaB protein from Thermotoga maritima. Characterization of an
            extremely thermophilic tRNA-methylthiotransferase
  JOURNAL   J Biol Chem 278 (32), 29515-29524 (2003)
   PUBMED   12766153
REFERENCE   2  (residues 1 to 474)
  AUTHORS   Pierrel,F., Bjork,G.R., Fontecave,M. and Atta,M.
  TITLE     Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein
  JOURNAL   J Biol Chem 277 (16), 13367-13370 (2002)
   PUBMED   11882645
REFERENCE   3  (residues 1 to 474)
  AUTHORS   Anantharaman,V., Koonin,E.V. and Aravind,L.
  TITLE     TRAM, a predicted RNA-binding domain, common to tRNA uracil
            methylation and adenine thiolation enzymes
  JOURNAL   FEMS Microbiol Lett 197 (2), 215-221 (2001)
   PUBMED   11313137
REFERENCE   4  (residues 1 to 474)
  AUTHORS   Esberg,B., Leung,H.C., Tsui,H.C., Bjork,G.R. and Winkler,M.E.
  TITLE     Identification of the miaB gene, involved in methylthiolation of
            isopentenylated A37 derivatives in the tRNA of Salmonella
            typhimurium and Escherichia coli
  JOURNAL   J Bacteriol 181 (23), 7256-7265 (1999)
   PUBMED   10572129
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR01574.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Vibrio"
                     /db_xref="taxon:662"
     gene            1..474
                     /gene="miaB"
     Protein         1..474
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /EC_number="2.8.4.3"
                     /GO_function="GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster
                     binding [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence
                     IEA]"
                     /calculated_mol_wt=53440
     Region          4..440
                     /region_name="miaB-methiolase"
                     /note="tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase
                     enzyme MiaB; TIGR01574"
                     /db_xref="CDD:273700"
ORIGIN      
        1 mskklliktw gcqmneydss kmadllnaan gyelteepee advlllntcs irekaqekvf
       61 hqlgrwktlk dkkpgvvigv ggcvatqegd hirerapfvd vifgpqtlhr lpemikqsqt
      121 ndapvmdisf peiekfdrlp epkadgataf vsimegcsky ctycvvpytr geevsrpmdd
      181 vlyeiaqlaa qgvrevnllg qnvnayrgpm hdgeicsfan llrlvasidg idrirfttsh
      241 pleftddiia vyedtpelvs flhlpvqsgs driltmmkrp htaieyksii rklrkarpei
      301 qissdfivgf pgetdkdfqd tmklikdvdf dmsfsfifsp rpgtpaadyp cdlsedakke
      361 rlyelqqtin aqamrysrlm lgteqrvlve gpskknlmel rartennrvv nfegsadlig
      421 qfvdvkivdv fanslrgelv rteqemdlrt visptqmmak trrenelgva tftp