hydroxyethylthiazole kinase [Listeria monocytogenes].

LOCUS       WP_010989417             269 aa            linear   BCT 11-MAR-2024
ACCESSION   WP_010989417
VERSION     WP_010989417.1
KEYWORDS    RefSeq.
SOURCE      Listeria monocytogenes
  ORGANISM  Listeria monocytogenes
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Listeriaceae;
            Listeria.
REFERENCE   1  (residues 1 to 269)
  AUTHORS   Drebes,J., Kunz,M., Windshugel,B., Kikhney,A.G., Muller,I.B.,
            Eberle,R.J., Oberthur,D., Cang,H., Svergun,D.I., Perbandt,M.,
            Betzel,C. and Wrenger,C.
  TITLE     Structure of ThiM from Vitamin B1 biosynthetic pathway of
            Staphylococcus aureus - Insights into a novel pro-drug approach
            addressing MRSA infections
  JOURNAL   Sci Rep 6, 22871 (2016)
   PUBMED   26960569
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 269)
  AUTHORS   Campobasso,N., Mathews,I.I., Begley,T.P. and Ealick,S.E.
  TITLE     Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase
            from Bacillus subtilis at 1.5 A resolution
  JOURNAL   Biochemistry 39 (27), 7868-7877 (2000)
   PUBMED   10891066
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF006830.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09355
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Listeria monocytogenes"
                     /db_xref="taxon:1639"
     gene            1..269
                     /gene="thiM"
     Protein         1..269
                     /product="hydroxyethylthiazole kinase"
                     /EC_number="2.7.1.50"
                     /GO_function="GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=27921
     Region          5..264
                     /region_name="PRK09355"
                     /note="hydroxyethylthiazole kinase; Validated"
                     /db_xref="CDD:236477"
     Site            order(22,24,30,34,40,42,62,64..65)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238575"
     Site            order(23..28,30..31,34,42..45,47..48,51..52,64..67,
                     100..101,187..190,238..239,241..243,245..246,249)
                     /site_type="other"
                     /note="multimerization interface [polypeptide binding]"
                     /db_xref="CDD:238575"
     Site            order(91,118,120,123,164,166,168,186,192,194)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238575"
ORIGIN      
        1 mfdfmtlekv rekgplvhni tnivvandsa ngllaigasp imasakeemd elakmadvlv
       61 inigtldgel vtamkiagra anvagtpvvl dpvgvgatsy rrkvvqella eiqfaairgn
      121 agelaaiage aweakgvdag vgsadvlsia gkvanewstv viisgevdvi sdgtrfakva
      181 ngsallprit gsgcllsavc gsfiavqdda frasveacas yavaseyaem elerklpgsf
      241 rplfldalas wsvektraka kiqesgehk