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MULTISPECIES: LacI family DNA-binding transcriptional regulator


LOCUS       WP_010989081             334 aa            linear   BCT 28-MAR-2023
            [Salmonella].
ACCESSION   WP_010989081
VERSION     WP_010989081.1
KEYWORDS    RefSeq.
SOURCE      Salmonella
  ORGANISM  Salmonella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae.
REFERENCE   1  (residues 1 to 334)
  AUTHORS   Bondos,S.E., Swint-Kruse,L. and Matthews,K.S.
  TITLE     Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox
            Proteins
  JOURNAL   J Biol Chem 290 (41), 24669-24677 (2015)
   PUBMED   26342073
REFERENCE   2  (residues 1 to 334)
  AUTHORS   Swint-Kruse,L. and Matthews,K.S.
  TITLE     Allostery in the LacI/GalR family: variations on a theme
  JOURNAL   Curr Opin Microbiol 12 (2), 129-137 (2009)
   PUBMED   19269243
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11265687
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Salmonella"
                     /db_xref="taxon:590"
     Protein         1..334
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /GO_function="GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription
                     factor activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /calculated_mol_wt=36030
     Region          10..79
                     /region_name="HTH_LACI"
                     /note="helix_turn _helix lactose operon repressor;
                     smart00354"
                     /db_xref="CDD:197675"
     Site            order(21..25,27..28,31,36..37,40,53,56,59..60,62..63)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143331"
     Site            53..62
                     /site_type="active"
                     /note="domain linker motif [active]"
                     /db_xref="CDD:143331"
     Region          69..333
                     /region_name="PBP1_GntR"
                     /note="ligand-binding domain of DNA transcription
                     repressor GntR specific for gluconate, a member of the
                     LacI-GalR family of bacterial transcription regulators;
                     cd01575"
                     /db_xref="CDD:380489"
     Site            order(77,83,86,90,99..102,115,118..119,121..122,226,228,
                     231,259)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380489"
     Site            order(81..82,85,130,153,166,200,225,278,295)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:380489"
ORIGIN      
        1 mkrtksprap tledvarsag lspmtvsral nspqlvrpkt vekvmqavrv tgyipnalag
       61 glasrrskli avvvpqinnn mfvdtiqsls delarrgyhi llcvagyteq teaelvatll
      121 srrpdgvvlt gihhtielkk vilnaaipvv eiwdltptpl dmlvgfshek vgqatgeyll
      181 skgyrrpgll wtadrraaqr kqglcsvlqr haihavpqvd vplpaslslg rsglsqlfde
      241 gtfdvivcss dtlaqgamme aesrglriph dlavigfgdl dfaasnrpsi ttvsvdrrai
      301 gqraatllad rieqkpcaea ivdigfhlig resa