bifunctional diguanylate cyclase/phosphodiesterase [Pseudomonas

LOCUS       WP_010895651             601 aa            linear   BCT 14-SEP-2023
            aeruginosa].
ACCESSION   WP_010895651
VERSION     WP_010895651.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (residues 1 to 601)
  AUTHORS   Ereno-Orbea,J., Oyenarte,I. and Martinez-Cruz,L.A.
  TITLE     CBS domains: Ligand binding sites and conformational variability
  JOURNAL   Arch Biochem Biophys 540 (1-2), 70-81 (2013)
   PUBMED   24161944
REFERENCE   2  (residues 1 to 601)
  AUTHORS   Tchigvintsev,A., Xu,X., Singer,A., Chang,C., Brown,G.,
            Proudfoot,M., Cui,H., Flick,R., Anderson,W.F., Joachimiak,A.,
            Galperin,M.Y., Savchenko,A. and Yakunin,A.F.
  TITLE     Structural insight into the mechanism of c-di-GMP hydrolysis by EAL
            domain phosphodiesterases
  JOURNAL   J Mol Biol 402 (3), 524-538 (2010)
   PUBMED   20691189
REFERENCE   3  (residues 1 to 601)
  AUTHORS   Schmidt,A.J., Ryjenkov,D.A. and Gomelsky,M.
  TITLE     The ubiquitous protein domain EAL is a cyclic diguanylate-specific
            phosphodiesterase: enzymatically active and inactive EAL domains
  JOURNAL   J Bacteriol 187 (14), 4774-4781 (2005)
   PUBMED   15995192
REFERENCE   4  (residues 1 to 601)
  AUTHORS   Pei,J. and Grishin,N.V.
  TITLE     GGDEF domain is homologous to adenylyl cyclase
  JOURNAL   Proteins 42 (2), 210-216 (2001)
   PUBMED   11119645
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 10112657
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Pseudomonas aeruginosa"
                     /db_xref="taxon:287"
     Protein         1..601
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /GO_function="GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /calculated_mol_wt=67099
     Region          27..259
                     /region_name="EAL"
                     /note="EAL domain. This domain is found in diverse
                     bacterial signaling proteins. It is called EAL after its
                     conserved residues and is also known as domain of unknown
                     function 2 (DUF2). The EAL domain has been shown to
                     stimulate degradation of a second...; cd01948"
                     /db_xref="CDD:238923"
     Region          290..410
                     /region_name="CBS_pair_SF"
                     /note="Two tandem repeats of the cystathionine
                     beta-synthase (CBS pair) domains superfamily; cl15354"
                     /db_xref="CDD:449531"
     Site            order(291,293..295,318..320,389,402,404,406..407,410)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341358"
     Region          291..359
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341358"
     Site            order(318,330,335..336,339,364,389..391,406)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341358"
     Region          364..410
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341358"
     Region          425..589
                     /region_name="GGDEF"
                     /note="Diguanylate-cyclase (DGC) or GGDEF domain; cd01949"
                     /db_xref="CDD:143635"
     Site            order(455,499)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143635"
     Site            order(460,463..464,468,472,495,497..500)
                     /site_type="active"
                     /db_xref="CDD:143635"
     Site            order(491,520)
                     /site_type="active"
                     /note="I-site [active]"
                     /db_xref="CDD:143635"
ORIGIN      
        1 mkygagcttg pfslrrersm tvteqlsald silahgdlhc lfqpilslse rrlvgyealt
       61 rgpsnsplhs plalfavarq cgrlselell crqrafsrfr diqgdamlfl nispesllea
      121 ghppgrtlql lqqlgispnr vvielteqtp iddfdlldta lhhyrsmgfs ialddlgagy
      181 sslrlwselr pdyvkidrhf vdgihldtvk refvgsilkm arasraqvia egielpeela
      241 vlsemgvdlv qgylfgrpqe hpasdvqqml psadapsqss sdehgdlral lleqravdvr
      301 tstqevveif rrqanlnsla ildeegrpig ivhrhaltda llrpfacdlf lrkpisrlms
      361 ddflavelgq tlqqvsrllt srarqrieed fivtrgghya gmgrvidvlr liteeklqqa
      421 rhanpltllp gnvpiqqcle rllqqrreav icyidvdsfk pfndlygyar gdevllclaq
      481 clseridpgs dfvghiggdd fmlvlgpedw rtridrllet fqsqcrrfys rehleagcfv
      541 ahnrhgqree ypllslsvgv vhlpaeacqg mdaahlatla seakrqakav pgyslhliea
      601 a