6-phosphofructokinase [Shigella flexneri].

LOCUS       WP_005089906             314 aa            linear   BCT 03-JUN-2024
ACCESSION   WP_005089906
VERSION     WP_005089906.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (residues 1 to 314)
  AUTHORS   Sakai,H. and Ohta,T.
  TITLE     Molecular cloning and nucleotide sequence of the gene for pyruvate
            kinase of Bacillus stearothermophilus and the production of the
            enzyme in Escherichia coli. Evidence that the genes for
            phosphofructokinase and pyruvate kinase constitute an operon
  JOURNAL   Eur J Biochem 211 (3), 851-859 (1993)
   PUBMED   8436141
REFERENCE   2  (residues 1 to 314)
  AUTHORS   Le Bras,G., Deville-Bonne,D. and Garel,J.R.
  TITLE     Purification and properties of the phosphofructokinase from
            Lactobacillus bulgaricus. A non-allosteric analog of the enzyme
            from Escherichia coli
  JOURNAL   Eur J Biochem 198 (3), 683-687 (1991)
   PUBMED   1828763
REFERENCE   3  (residues 1 to 314)
  AUTHORS   Hellinga,H.W. and Evans,P.R.
  TITLE     Mutations in the active site of Escherichia coli
            phosphofructokinase
  JOURNAL   Nature 327 (6121), 437-439 (1987)
   PUBMED   2953977
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR02482.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /db_xref="taxon:623"
     gene            1..314
                     /gene="pfkA"
     Protein         1..314
                     /product="6-phosphofructokinase"
                     /EC_number="2.7.1.11"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /calculated_mol_wt=34072
     Region          2..313
                     /region_name="PRK03202"
                     /note="ATP-dependent 6-phosphofructokinase"
                     /db_xref="CDD:235111"
     Site            order(6,36,67,98..100,102..103,120,122,124,164..166,217,
                     244,247)
                     /site_type="active"
                     /db_xref="CDD:238388"
     Site            order(6,36,67,98..100,102..103)
                     /site_type="other"
                     /note="ADP/pyrophosphate binding site [chemical binding]"
                     /db_xref="CDD:238388"
     Site            order(16,20,49,54,57,130,142,146,149,177..178,180,208,
                     256..257,261,268,283,312..314)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238388"
     Site            order(16,20,49..50,53..54,149,180,182,206,208..210)
                     /site_type="active"
                     /note="allosteric effector site [active]"
                     /db_xref="CDD:238388"
     Site            order(120,122,124,157,164..166,217,238,244,247)
                     /site_type="other"
                     /note="fructose-1,6-bisphosphate binding site"
                     /db_xref="CDD:238388"
ORIGIN      
        1 mltsggdapg mnaairgvvr salteglevm giydgylgly edrmvqldry svsdminrgg
       61 tflgsarfpe frdenirava ienlkkrgid alvviggdgs ymgamrltem gfpciglpgt
      121 idndikgtdy tigfftalst vveaidrlrd tssshqrisv vevmgrycgd ltlaaaiagg
      181 cefvvvpeve fsredlvnei kagiakgkkh aivaitehmc dvdelahfie ketgretrat
      241 vlghiqrggs pvpydrilas rmgayaidll lagyggrcvg iqneqlvhhd iidaienmkr
      301 pfkgdwldca kkly