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LOCUS WP_004733414 562 aa linear BCT 31-MAR-2023 ACCESSION WP_004733414 VERSION WP_004733414.1 KEYWORDS RefSeq. SOURCE Vibrio ORGANISM Vibrio Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae. REFERENCE 1 (residues 1 to 562) AUTHORS Liu,Y., Guo,X., Liu,W., Wang,J. and Kent Zhao,Z. TITLE Structural Insights into Malic Enzyme Variants Favoring an Unnatural Redox Cofactor JOURNAL Chembiochem 22 (10), 1765-1768 (2021) PUBMED 33523590 REFERENCE 2 (residues 1 to 562) AUTHORS Burley,K.H., Cuthbert,B.J., Basu,P., Newcombe,J., Irimpan,E.M., Quechol,R., Foik,I.P., Mobley,D.L., Beste,D.J.V. and Goulding,C.W. TITLE Structural and Molecular Dynamics of Mycobacterium tuberculosis Malic Enzyme, a Potential Anti-TB Drug Target JOURNAL ACS Infect Dis 7 (1), 174-188 (2021) PUBMED 33356117 REFERENCE 3 (residues 1 to 562) AUTHORS Bologna,F.P., Andreo,C.S. and Drincovich,M.F. TITLE Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure JOURNAL J Bacteriol 189 (16), 5937-5946 (2007) PUBMED 17557829 REFERENCE 4 (residues 1 to 562) AUTHORS Wang,J., Tan,H. and Zhao,Z.K. TITLE Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12 JOURNAL Protein Expr Purif 53 (1), 97-103 (2007) PUBMED 17215140 REFERENCE 5 (residues 1 to 562) AUTHORS Yang,Z., Zhang,H., Hung,H.C., Kuo,C.C., Tsai,L.C., Yuan,H.S., Chou,W.Y., Chang,G.G. and Tong,L. TITLE Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme JOURNAL Protein Sci 11 (2), 332-341 (2002) PUBMED 11790843 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 11486672 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..562 /organism="Vibrio" /db_xref="taxon:662" Protein 1..562 /product="NAD-dependent malic enzyme" /EC_number="1.1.1.38" /GO_function="GO:0004470 - malic enzyme activity [Evidence IEA]" /GO_function="GO:0004471 - malate dehydrogenase (decarboxylating) (NAD+) activity [Evidence IEA]" /GO_function="GO:0046872 - metal ion binding [Evidence IEA]" /GO_function="GO:0051287 - NAD binding [Evidence IEA]" /calculated_mol_wt=61863 Region 1..560 /region_name="PRK13529" /note="oxaloacetate-decarboxylating malate dehydrogenase" /db_xref="CDD:237414" ORIGIN 1 mnndkrplyi pyagpallst pllnkgsafs aeerssfnle gllpetteti qeqvgraykq 61 ycnfesdmdk hiylrniqdt netlfyrlvq nhisemmpii ytptvgaace nfsniyrrgr 121 glfisypnrd riddllnnat nhnvkvivvt dgerilglgd qgiggmgipi gklalytacg 181 gispaymlpi vldvgtnnpq rladpmymgw rhpritgady dafveefiqa vqrrwpdalv 241 qfedfaqkna mpllerykdr lccfnddiqg taavtvgsll aackaanskl sdqritflga 301 gsagcgiaea iiaqmvsegi sdaqarsqvy mvdrwgllqe gmqnlldfqq rlvqtnentk 361 dwesdgtgfs lldvvrhakp tvlvgvsgap glfskevike mnlhcerpiv fplsnptsrv 421 eatpndiirw tdgqalvatg spfepvthng ttypiaqcnn syifpgiglg vlavnasrit 481 demlmessra latcsplain gsgallpple eihtvskkia lavgkkaieq gvaleiteea 541 lqqaidqhfw qpvyrrykrt af