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MULTISPECIES: LacI family DNA-binding transcriptional regulator


LOCUS       WP_004229436             333 aa            linear   BCT 20-NOV-2023
            [Klebsiella].
ACCESSION   WP_004229436
VERSION     WP_004229436.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella
  ORGANISM  Klebsiella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group.
REFERENCE   1  (residues 1 to 333)
  AUTHORS   Bondos,S.E., Swint-Kruse,L. and Matthews,K.S.
  TITLE     Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox
            Proteins
  JOURNAL   J Biol Chem 290 (41), 24669-24677 (2015)
   PUBMED   26342073
REFERENCE   2  (residues 1 to 333)
  AUTHORS   Swint-Kruse,L. and Matthews,K.S.
  TITLE     Allostery in the LacI/GalR family: variations on a theme
  JOURNAL   Curr Opin Microbiol 12 (2), 129-137 (2009)
   PUBMED   19269243
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11265705
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Klebsiella"
                     /db_xref="taxon:570"
     Protein         1..333
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /GO_function="GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription
                     factor activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /calculated_mol_wt=36965
     Region          1..67
                     /region_name="HTH_LACI"
                     /note="helix_turn _helix lactose operon repressor;
                     smart00354"
                     /db_xref="CDD:197675"
     Site            order(10..14,16..17,20,25..26,29,42,45,48..49,51..52)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143331"
     Site            42..51
                     /site_type="active"
                     /note="domain linker motif [active]"
                     /db_xref="CDD:143331"
     Region          58..324
                     /region_name="PBP1_GalS-like"
                     /note="ligand binding domain of DNA transcription
                     iso-repressor GalS, which is one of two regulatory
                     proteins involved in galactose transport and metabolism;
                     cd06270"
                     /db_xref="CDD:380494"
     Site            order(58,65,71,76,79,88..91,93,107,111,219..220,252,
                     278..279)
                     /site_type="other"
                     /note="dimer interface (closed form) [polypeptide
                     binding]"
                     /db_xref="CDD:380494"
     Site            order(70,119,192,217,243,271)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380494"
ORIGIN      
        1 mldvsiragv skatvsrvln gtgqvkestr qqvfkameel gyrpnflars lanqtsnsig
       61 lvvstfdgfy fgrllqqasr qteawgkqli vtdghdtper eeeavqmlad rqcdaiilyt
      121 rhmsekaims linsiamplv vinrdvaqar ercvffeqqe aafqaveyli tqghrdiaci
      181 tvpmhtptgq arlqgyrnal ikhgiewdps rvkygdstmt rgyelcrell dekarfsalf
      241 scnddmalga skalhqaglr ipqdvslfgf ddapsakwle pglstvylpi dnmittaidq
      301 aiklanqqpi etippftgtl vlresvttgp ffk