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LOCUS WP_004200028 565 aa linear BCT 31-MAR-2023 ACCESSION WP_004200028 VERSION WP_004200028.1 KEYWORDS RefSeq. SOURCE Klebsiella ORGANISM Klebsiella Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group. REFERENCE 1 (residues 1 to 565) AUTHORS Liu,Y., Guo,X., Liu,W., Wang,J. and Kent Zhao,Z. TITLE Structural Insights into Malic Enzyme Variants Favoring an Unnatural Redox Cofactor JOURNAL Chembiochem 22 (10), 1765-1768 (2021) PUBMED 33523590 REFERENCE 2 (residues 1 to 565) AUTHORS Burley,K.H., Cuthbert,B.J., Basu,P., Newcombe,J., Irimpan,E.M., Quechol,R., Foik,I.P., Mobley,D.L., Beste,D.J.V. and Goulding,C.W. TITLE Structural and Molecular Dynamics of Mycobacterium tuberculosis Malic Enzyme, a Potential Anti-TB Drug Target JOURNAL ACS Infect Dis 7 (1), 174-188 (2021) PUBMED 33356117 REFERENCE 3 (residues 1 to 565) AUTHORS Bologna,F.P., Andreo,C.S. and Drincovich,M.F. TITLE Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure JOURNAL J Bacteriol 189 (16), 5937-5946 (2007) PUBMED 17557829 REFERENCE 4 (residues 1 to 565) AUTHORS Wang,J., Tan,H. and Zhao,Z.K. TITLE Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12 JOURNAL Protein Expr Purif 53 (1), 97-103 (2007) PUBMED 17215140 REFERENCE 5 (residues 1 to 565) AUTHORS Yang,Z., Zhang,H., Hung,H.C., Kuo,C.C., Tsai,L.C., Yuan,H.S., Chou,W.Y., Chang,G.G. and Tong,L. TITLE Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme JOURNAL Protein Sci 11 (2), 332-341 (2002) PUBMED 11790843 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 11486672 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..565 /organism="Klebsiella" /db_xref="taxon:570" Protein 1..565 /product="NAD-dependent malic enzyme" /EC_number="1.1.1.38" /GO_function="GO:0004470 - malic enzyme activity [Evidence IEA]" /GO_function="GO:0004471 - malate dehydrogenase (decarboxylating) (NAD+) activity [Evidence IEA]" /GO_function="GO:0046872 - metal ion binding [Evidence IEA]" /GO_function="GO:0051287 - NAD binding [Evidence IEA]" /calculated_mol_wt=62655 Region 1..563 /region_name="PRK13529" /note="oxaloacetate-decarboxylating malate dehydrogenase" /db_xref="CDD:237414" ORIGIN 1 mqfthkknrs lyipyagpvl lefpllnkgs afsmeersnf nllgllpevv etieeqaera 61 wiqyqgfkte idkhiylrni qdtnetlfyr lignhleemm pviytptvga acerfseiyr 121 rargvfisyq nrhnlddilq nvpnhnvkvi vvtdgerilg lgdqgiggmg ipigklslyt 181 tcggispayt lpivldvgtn nqqllddply mgwrhpritd deyyqfvddv iqaikarwpd 241 vllqfedfaq knampllnry rneicsfndd iqgtaavtvg tliaasrgag sqlseqkivf 301 lgagsagcgi aeqiiaqivr eglseeearq rvfmvdrfgl ltdgmpnllp fqnklvqkre 361 qlqswdttse alsllevvrn vkpniligvs gqpglfteei iremhkhcpr pivmplsnpt 421 srveatpqni lswtdgealv atgspfspvt vkgkqypiaq cnnsyifpgi glgviasgas 481 rvtdemlmaa setlaqhspl vnngegpvlp elkdiqtvsr aiafavgkva qeqgvavkts 541 aeallqaisd nfwlpeyrny rrtsi