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MULTISPECIES: alpha-galactosidase [Klebsiella].


LOCUS       WP_004151861             707 aa            linear   BCT 31-DEC-2024
ACCESSION   WP_004151861
VERSION     WP_004151861.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella
  ORGANISM  Klebsiella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group.
REFERENCE   1  (residues 1 to 707)
  AUTHORS   Coines,J., Raich,L. and Rovira,C.
  TITLE     Modeling catalytic reaction mechanisms in glycoside hydrolases
  JOURNAL   Curr Opin Chem Biol 53, 183-191 (2019)
   PUBMED   31731209
REFERENCE   2  (residues 1 to 707)
  AUTHORS   Naumoff,D.G.
  TITLE     Hierarchical classification of glycoside hydrolases
  JOURNAL   Biochemistry (Mosc) 76 (6), 622-635 (2011)
   PUBMED   21639842
REFERENCE   3  (residues 1 to 707)
  AUTHORS   Davies,G. and Henrissat,B.
  TITLE     Structures and mechanisms of glycosyl hydrolases
  JOURNAL   Structure 3 (9), 853-859 (1995)
   PUBMED   8535779
REFERENCE   4  (residues 1 to 707)
  AUTHORS   Henrissat,B., Callebaut,I., Fabrega,S., Lehn,P., Mornon,J.P. and
            Davies,G.
  TITLE     Conserved catalytic machinery and the prediction of a common fold
            for several families of glycosyl hydrolases
  JOURNAL   Proc Natl Acad Sci U S A 92 (15), 7090-7094 (1995)
   PUBMED   7624375
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1996 May 28;93(11):5674. doi:
            10.1073/pnas.93.11.5674. PMID: 8643635]
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 16044983
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Klebsiella"
                     /db_xref="taxon:570"
     Protein         1..707
                     /product="alpha-galactosidase"
                     /EC_number="3.2.1.22"
                     /GO_function="GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /GO_process="GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /calculated_mol_wt=79802
     Region          24..232
                     /region_name="Glyco_hydro_36N"
                     /note="Glycosyl hydrolase family 36 N-terminal domain;
                     pfam16875"
                     /db_xref="CDD:465291"
     Region          253..592
                     /region_name="AmyAc_family"
                     /note="Alpha amylase catalytic domain family; cl38930"
                     /db_xref="CDD:476817"
     Site            order(299,330,374,439,443,483,502,505)
                     /site_type="active"
                     /db_xref="CDD:269892"
     Region          609..692
                     /region_name="Glyco_hydro_36C"
                     /note="Glycosyl hydrolase family 36 C-terminal domain;
                     pfam16874"
                     /db_xref="CDD:465290"
ORIGIN      
        1 msdslihlqs agadvviktr pfaeivywgp hlshfspqda dsltrpvang rldvdspvtl
       61 maelghglfg apgieghrqg ldasplftts evrhegqtlt lvsedpqagl rlqseialda
      121 sgvlsvrhgv tnlraspwqv drlavtlpva erarevmafh grwirefqph rltlehdsfv
      181 lenrrgrtsh ehfpalitgs rafsemqgev wgvhlawsgn hrlraevktd grrylqaeal
      241 ylpgemalae getlwtpyly asysanglng msqqfhrylr eriirfpgnk prpvhlntwe
      301 giyfdhdpdy imrmadeaaa lgverfiidd gwfkgrnddw aalgdwylde kkypygltpv
      361 idhvkslgme fgiwvepemi npdsdlyrah pdwvlalpgy tpltgrhqfv lnlnipeafd
      421 yllermswll gehavdyvkw dmnrelvqpg hqgraaadaq trqfyrlldt lvarfphief
      481 escssgggri dyevlkrshr fwasdnndal erntiqrgms yffppevmga hignrhchat
      541 frqhsiafrg ltalfghmgl eldpvsadee eragyrkyaa lhkqwrdvih hgvqwridmp
      601 dattlahgvv spdkaqaifl vsqlampdyt lmaplrlagl easaryqvtl ldhpniqitg
      661 egghtmrklp awmttpqtvs gewlqqagla lpildpesai liglqrv