MULTISPECIES: tRNA 2-thiouridine(34) synthase MnmA [Klebsiella].

LOCUS       WP_004150803             368 aa            linear   BCT 27-DEC-2023
ACCESSION   WP_004150803
VERSION     WP_004150803.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella
  ORGANISM  Klebsiella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group.
REFERENCE   1  (residues 1 to 368)
  AUTHORS   Numata,T., Ikeuchi,Y., Fukai,S., Suzuki,T. and Nureki,O.
  TITLE     Snapshots of tRNA sulphuration via an adenylated intermediate
  JOURNAL   Nature 442 (7101), 419-424 (2006)
   PUBMED   16871210
REFERENCE   2  (residues 1 to 368)
  AUTHORS   Kambampati,R. and Lauhon,C.T.
  TITLE     MnmA and IscS are required for in vitro 2-thiouridine biosynthesis
            in Escherichia coli
  JOURNAL   Biochemistry 42 (4), 1109-1117 (2003)
   PUBMED   12549933
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00420.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Klebsiella"
                     /db_xref="taxon:570"
     gene            1..368
                     /gene="mnmA"
                     /gene_synonym="trmU"
     Protein         1..368
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /EC_number="2.8.1.13"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /calculated_mol_wt=40885
     Region          6..365
                     /region_name="MnmA"
                     /note="tRNA U34 2-thiouridine synthase MnmA/TrmU, contains
                     the PP-loop ATPase domain [Translation, ribosomal
                     structure and biogenesis]; COG0482"
                     /db_xref="CDD:440250"
ORIGIN      
        1 msesqkkviv gmsggvdssv saylllqqgy kveglfmknw eeddgeeyct aaadladaqa
       61 vcdklgielh tvnfaaeywd nvfelfleey kagrtpnpdi lcnkeikfka flefaaedlg
      121 adyiatghyv rradvdgksq llrgldgnkd qsyflytlsh eqiaqslfpv gelekpqvrk
      181 iaeeldlita kkkdstgicf igerkfrdfl grylpaqpgk iltvdgeeig thqglmyhtl
      241 gqrkglgigg tkegsedpwy vvdkdvenni livaqghdhp rlmsvgliaq qlhwvnrepl
      301 qgtlrctvkt ryrqtdipct vtaldedrie vrfdepvaav tpgqsavfyl gevclgggii
      361 eqrlplqs