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LOCUS WP_003732553 403 aa linear BCT 24-APR-2023 ACCESSION WP_003732553 VERSION WP_003732553.1 KEYWORDS RefSeq. SOURCE Listeria monocytogenes ORGANISM Listeria monocytogenes Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Listeriaceae; Listeria. REFERENCE 1 (residues 1 to 403) AUTHORS Martinez-Gomez,N.C., Palmer,L.D., Vivas,E., Roach,P.L. and Downs,D.M. TITLE The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica JOURNAL J Bacteriol 193 (18), 4582-4587 (2011) PUBMED 21724998 REFERENCE 2 (residues 1 to 403) AUTHORS You,D., Xu,T., Yao,F., Zhou,X. and Deng,Z. TITLE Direct evidence that ThiI is an ATP pyrophosphatase for the adenylation of uridine in 4-thiouridine biosynthesis JOURNAL Chembiochem 9 (12), 1879-1882 (2008) PUBMED 18604845 REFERENCE 3 (residues 1 to 403) AUTHORS Mueller,E.G., Palenchar,P.M. and Buck,C.J. TITLE The role of the cysteine residues of ThiI in the generation of 4-thiouridine in tRNA JOURNAL J Biol Chem 276 (36), 33588-33595 (2001) PUBMED 11443125 REFERENCE 4 (residues 1 to 403) AUTHORS Webb,E., Claas,K. and Downs,D.M. TITLE Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium JOURNAL J Bacteriol 179 (13), 4399-4402 (1997) PUBMED 9209060 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: HMM Evidence Accession :: TIGR00342.1 Evidence Source :: JCVI ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..403 /organism="Listeria monocytogenes" /db_xref="taxon:1639" gene 1..403 /gene="thiI" Protein 1..403 /product="tRNA uracil 4-sulfurtransferase ThiI" /EC_number="2.8.1.4" /GO_function="GO:0140741 - tRNA U4 sulfurtransferase [Evidence IEA]" /GO_process="GO:0006400 - tRNA modification [Evidence IEA]" /GO_process="GO:0009228 - thiamine biosynthetic process [Evidence IEA]" /calculated_mol_wt=44843 Region 3..384 /region_name="ThiI" /note="Adenylyl- and sulfurtransferase ThiI (thiamine and tRNA 4-thiouridine biosynthesis) [Coenzyme transport and metabolism, Translation, ribosomal structure and biogenesis]; COG0301" /db_xref="CDD:440070" ORIGIN 1 mefdrmliry gelstkgknr kqfvtklaqn vkramtdlpe vrihgerdrm yiilngadyq 61 laeerlkpif giqsfspavr vnldveevka aalalvqdah eengtfkvaa rrshrefpld 121 sneinqeiga yvlqnmedlt vnvknpdvkl tidvrkegvf lscrtilgaa glpvgssgra 181 mlmlsggids pvagylaqkr gveieavhfh sppytseqak qkaidlaakl akysgqvqmh 241 ivpfteiqev ikqqipesvi mtvtrrmmlr itdelrrkrn glaivngesl gqvasqtles 301 mlainavtat piirpvvsmd kneiiqiaqk idtynlsvqp fedcctiftp pspktkpkld 361 kiehyesftd fdaliakald nietisvnva etaqvkdefa dlf