MULTISPECIES: paerucumarin biosynthesis oxygenase PvcB

LOCUS       WP_003115812             291 aa            linear   BCT 20-NOV-2023
            [Pseudomonas].
ACCESSION   WP_003115812
VERSION     WP_003115812.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 291)
  AUTHORS   Zhu,J., Lippa,G.M., Gulick,A.M. and Tipton,P.A.
  TITLE     Examining Reaction Specificity in PvcB, a Source of Diversity in
            Isonitrile-Containing Natural Products
  JOURNAL   Biochemistry 54 (16), 2659-2669 (2015)
   PUBMED   25866990
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR009572
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     gene            1..291
                     /gene="pvcB"
     Protein         1..291
                     /product="paerucumarin biosynthesis oxygenase PvcB"
                     /calculated_mol_wt=33006
     Region          14..280
                     /region_name="CAS_like"
                     /note="Clavaminic acid synthetase (CAS) -like; CAS is a
                     trifunctional Fe(II)/ 2-oxoglutarate (2OG) oxygenase
                     carrying out three reactions in the biosynthesis of
                     clavulanic acid, an inhibitor of class A serine
                     beta-lactamases. In general, Fe(II)-2OG oxygenases...;
                     cd00250"
                     /db_xref="CDD:238154"
     Site            order(79..80,113,115,120,169,274,276)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238154"
     Site            order(110,112,139,259,270)
                     /site_type="active"
                     /db_xref="CDD:238154"
     Site            order(110,112,259)
                     /site_type="other"
                     /note="iron coordination sites [ion binding]"
                     /db_xref="CDD:238154"
ORIGIN      
        1 mnaylsdqpv rlsplrdeqg nqprfgllle pgrpgmhvge lpaqwlkgla rshhllllrg
       61 faafadaesl trychdfgev mlwpfgavle lveqegaedh ifannyvplh wdgmyletvp
      121 efqvfhcvda pgdsdggrtt fsstpaalql adsselelwr rasgryqrsa ahyssrsaap
      181 iverhprref pilrfceppv egdasfinps efhydgiape qrgellaslr rclyhpqahy
      241 ahrwrsddlv iadnltllhg reafahrapr hlrrvhihae palrnphlqr d