MULTISPECIES: T3SS effector bifunctional cytotoxin exoenzyme T

LOCUS       WP_003114583             457 aa            linear   BCT 20-NOV-2023
            [Pseudomonas].
ACCESSION   WP_003114583
VERSION     WP_003114583.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR008173
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     gene            1..457
                     /gene="exoT"
     Protein         1..457
                     /product="T3SS effector bifunctional cytotoxin exoenzyme
                     T"
                     /calculated_mol_wt=48384
     Region          108..227
                     /region_name="ToxGAP"
                     /note="GTPase-activating protein (GAP) domain found in
                     bacterial cytotoxins, ExoS, SptP, and YopE. Part of
                     protein secretion system; stimulates Rac1- dependent
                     cytoskeletal changes that promote bacterial
                     internalization; cd00219"
                     /db_xref="CDD:119405"
     Site            order(122,143..145,150,153)
                     /site_type="active"
                     /note="switch II binding region [active]"
                     /db_xref="CDD:119405"
     Site            order(144,149)
                     /site_type="other"
                     /note="Rac1 P-loop interaction site [polypeptide binding]"
                     /db_xref="CDD:119405"
     Site            order(149,189..191)
                     /site_type="other"
                     /note="GTP binding residues [chemical binding]"
                     /db_xref="CDD:119405"
     Site            order(149,153,186)
                     /site_type="active"
                     /note="switch I binding region [active]"
                     /db_xref="CDD:119405"
     Region          248..433
                     /region_name="VIP2"
                     /note="A family of actin-ADP-ribosylating toxin. A member
                     of the Bacillus-prodiced vegetative insecticidal proteins
                     (VIPs) possesses high specificity against the major insect
                     pest, corn rootworms, and belongs to a classs of binary
                     toxins and regulators of...; cl00173"
                     /db_xref="CDD:469640"
     Site            order(275,288,322,346..348,360,385,394)
                     /site_type="active"
                     /db_xref="CDD:238144"
     Site            288
                     /site_type="active"
                     /note="conformational flexibility of ligand binding pocket
                     [active]"
                     /db_xref="CDD:238144"
     Site            order(380,383,385)
                     /site_type="active"
                     /note="ADP-ribosylating toxin turn-turn motif [active]"
                     /db_xref="CDD:238144"
ORIGIN      
        1 mhiqssqqnp sfvaelsqav agrlgqvear qvatpreaqq laqrqeapkg egllsrlgaa
       61 larpfvaiie wlgkllgsra haatqaplsr qdappaasls aaeikqmmlq kalpltlggl
      121 gkaselatlt aerlakdhtr lasgdgalrs latalvgird gsrieasrtq aarlleqsvg
      181 gialqqwgta ggaasqhvls aspeqlreia vqlhavmdkv allrhavese vkgepvdkal
      241 adglvehfgl eaeqylgehp dgpysdaevm alglytngey qhlnrslrqg reldagqali
      301 drgmsaafek sgpaeqvvkt frgtqgrdaf eavkegqvgh dagylstsrd pgvarsfagq
      361 gtittlfgrs gidvseisie gdeqeilydk gtdmrvllsa kdgqgvtrrv leeatlgers
      421 ghgeglldal dlatgtdrsg kpqeqdlrlr mrgldla