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LOCUS WP_003114402 1146 aa linear BCT 31-DEC-2024 ACCESSION WP_003114402 VERSION WP_003114402.1 KEYWORDS RefSeq. SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (residues 1 to 1146) AUTHORS Gao,Y., Cao,D., Zhu,J., Feng,H., Luo,X., Liu,S., Yan,X.X., Zhang,X. and Gao,P. TITLE Structural insights into assembly, operation and inhibition of a type I restriction-modification system JOURNAL Nat Microbiol 5 (9), 1107-1118 (2020) PUBMED 32483229 REFERENCE 2 (residues 1 to 1146) AUTHORS Loenen,W.A., Dryden,D.T., Raleigh,E.A. and Wilson,G.G. TITLE Type I restriction enzymes and their relatives JOURNAL Nucleic Acids Res 42 (1), 20-44 (2014) PUBMED 24068554 REFERENCE 3 (residues 1 to 1146) AUTHORS Gustafson,E.A. and Wessel,G.M. TITLE DEAD-box helicases: posttranslational regulation and function JOURNAL Biochem Biophys Res Commun 395 (1), 1-6 (2010) PUBMED 20206133 REFERENCE 4 (residues 1 to 1146) AUTHORS Roberts,R.J., Belfort,M., Bestor,T., Bhagwat,A.S., Bickle,T.A., Bitinaite,J., Blumenthal,R.M., Degtyarev,S.Kh., Dryden,D.T., Dybvig,K., Firman,K., Gromova,E.S., Gumport,R.I., Halford,S.E., Hattman,S., Heitman,J., Hornby,D.P., Janulaitis,A., Jeltsch,A., Josephsen,J., Kiss,A., Klaenhammer,T.R., Kobayashi,I., Kong,H., Kruger,D.H., Lacks,S., Marinus,M.G., Miyahara,M., Morgan,R.D., Murray,N.E., Nagaraja,V., Piekarowicz,A., Pingoud,A., Raleigh,E., Rao,D.N., Reich,N., Repin,V.E., Selker,E.U., Shaw,P.C., Stein,D.C., Stoddard,B.L., Szybalski,W., Trautner,T.A., Van Etten,J.L., Vitor,J.M., Wilson,G.G. and Xu,S.Y. TITLE A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes JOURNAL Nucleic Acids Res 31 (7), 1805-1812 (2003) PUBMED 12654995 REFERENCE 5 (residues 1 to 1146) AUTHORS Piekarowicz,A., Klyz,A., Kwiatek,A. and Stein,D.C. TITLE Analysis of type I restriction modification systems in the Neisseriaceae: genetic organization and properties of the gene products JOURNAL Mol Microbiol 41 (5), 1199-1210 (2001) PUBMED 11555298 REFERENCE 6 (residues 1 to 1146) AUTHORS Makovets,S., Doronina,V.A. and Murray,N.E. TITLE Regulation of endonuclease activity by proteolysis prevents breakage of unmodified bacterial chromosomes by type I restriction enzymes JOURNAL Proc Natl Acad Sci U S A 96 (17), 9757-9762 (1999) PUBMED 10449767 REFERENCE 7 (residues 1 to 1146) AUTHORS Murray,N.E., Daniel,A.S., Cowan,G.M. and Sharp,P.M. TITLE Conservation of motifs within the unusually variable polypeptide sequences of type I restriction and modification enzymes JOURNAL Mol Microbiol 9 (1), 133-143 (1993) PUBMED 8412658 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 11427405 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..1146 /organism="Pseudomonas aeruginosa" /db_xref="taxon:287" Protein 1..1146 /product="type I restriction endonuclease subunit R" /EC_number="3.1.21.3" /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_function="GO:0004386 - helicase activity [Evidence IEA]" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_function="GO:0009035 - type I site-specific deoxyribonuclease activity [Evidence IEA]" /GO_function="GO:0016887 - ATP hydrolysis activity [Evidence IEA]" /GO_process="GO:0009307 - DNA restriction-modification system [Evidence IEA]" /calculated_mol_wt=128541 Region 4..1007 /region_name="COG0610" /note="Type I site-specific restriction-modification system, R (restriction) subunit and related helicases ... [Defense mechanisms]" /db_xref="CDD:440375" ORIGIN 1 mkptdtseka leqlivrhla gisehasvpp navqspgaiy alggyvlgra tdfnrdvald 61 tvqllaflqa tqpdavetle lahegikrtq flhrlqgeit krgvvdvlrk gvshgpvrvd 121 lyrllptpgn tnaaeafgrn ifsitrqvry shdsgneldl vifvnglpvl tfelknsltk 181 qtvadaivqy qstrspqell fqpgrciahf avddaevyfc telkgkaswf lpfnqgwnng 241 agnppnpngl ktdylwkqvl akeslaniie sfaqvveeqe tdattgrkkk krkpvfprfh 301 qlrtvrallr rtrsdgvgkr yliqhsagsg ksntiawlah qlvelrsvad plmpqfdsii 361 vitdrraldt qiartikgyd hvasifghsd naqelrdylr rgrkiivttv qkfpfileei 421 gdlsgrtfal lideahssqg gkttarmhea lsgktdeeaf eedatqdavn teiekriqsr 481 rmlsnasyfa ftatpknrtl elfgektvvd dkvefrspee ltyttkqaiq egfildviah 541 yttldsfyqv aktvendpdf dkaralkkir hyveshdkai rrkaeimvdh fvaqvmgakk 601 iggkaramiv cngiaraidy wrevsdyleq ikspykaiva ysgefeigge kkteadlndf 661 pskeipdrlr qdpyrfliva nkfvtgfdep llhtmyvdkk lagvqavqtl srlnrahpqk 721 hdtfvldfad naeavkkafq dyyratiqtg etdanklhdl kadlddkqiy swpqvedlva 781 lylsgadrdk ldpildhcve qyqanldedn qvefkgkaka fvrsygflaa ilsyghpawe 841 klsiflnfli pklpapkeed lskgvlesid idsyrveara alkmamddtd atvevapvgg 901 gggkgepdid klsnilktfn dlfgnidwkd adkigkviae eipvrvaqdk ayqnaqvnsd 961 rqnaklehdk alnrvvlell sdhtelfkqf sdnpnfkrwl tdmvfdvtyq qnpahgvedv 1021 rmraldivrd rfdaavvwsq avdmlldrla aggrnslgia dlalieetsg lsvshvlfpv 1081 lnllsahdvg ilnrefvrvg stdtpqtmsf devrllaqkd pegddwaecl sirwalqdaq 1141 riahgr