pyruvate kinase [Pseudomonas aeruginosa].

LOCUS       WP_003114299             477 aa            linear   BCT 19-NOV-2024
ACCESSION   WP_003114299
VERSION     WP_003114299.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (residues 1 to 477)
  AUTHORS   Zhong,W., Morgan,H.P., McNae,I.W., Michels,P.A.,
            Fothergill-Gilmore,L.A. and Walkinshaw,M.D.
  TITLE     `In crystallo' substrate binding triggers major domain movements
            and reveals magnesium as a co-activator of Trypanosoma brucei
            pyruvate kinase
  JOURNAL   Acta Crystallogr D Biol Crystallogr 69 (Pt 9), 1768-1779 (2013)
   PUBMED   23999300
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR01064.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Pseudomonas aeruginosa"
                     /db_xref="taxon:287"
     gene            1..477
                     /gene="pyk"
     Protein         1..477
                     /product="pyruvate kinase"
                     /EC_number="2.7.1.40"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0004743 - pyruvate kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030955 - potassium ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /calculated_mol_wt=51414
     Region          1..476
                     /region_name="PRK06247"
                     /note="pyruvate kinase; Provisional"
                     /db_xref="CDD:180487"
     Site            order(6..7,63,65,73..76,159..160,166,169..173,188,196,203,
                     247,265..266,270,302,304,333,335..336,388,390,392,403,
                     406..407,409,411..413)
                     /site_type="active"
                     /note="domain interfaces [active]"
                     /db_xref="CDD:238178"
     Site            order(35,37,69,194,217,219,243,275)
                     /site_type="active"
                     /db_xref="CDD:238178"
ORIGIN      
        1 mtadkkakil atlgpatrsr ddiralveag anllrlnfsh gdyadhaqrf awvreveael
       61 nypigvlmdl qgpklrvgrf aagavqlqrg qtftldlsda pgderrvnlp hpeiihalep
      121 gmslllddgk irlevvnchs daietrvavg gelsdrkgvn vpeavlqlsp ltdkdrrdla
      181 fglelgvdwv alsfvqrped ideargligd kaflmakiek psavsaieai aeradaimva
      241 rgdlgvevpa esvpgiqkri vqvcrqlgkp vvvatqmles mrfspaptra evtdvatavg
      301 agadavmlsa etasgqypre avemmakivr qveaepdyhv qlevnrpqpd atvsdaisca
      361 irrvsrilpv avlvnytesg nstlraarer pkapilsltp nlrtarrltv awgvysvvne
      421 qlahvdeics taldialaqr marrgdtvvv tagvpfgrpg stnmlrietv applgdl