chlorophyllase/cutinase-like alpha/beta fold protein [Pseudomonas

LOCUS       WP_003113944             359 aa            linear   BCT 02-MAR-2025
            aeruginosa].
ACCESSION   WP_003113944
VERSION     WP_003113944.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (residues 1 to 359)
  AUTHORS   Zhang,Z., Wang,W., Li,D., Xiao,J., Wu,L., Geng,X., Wu,G., Zeng,Z.
            and Hu,J.
  TITLE     Decolorization of molasses alcohol wastewater by thermophilic
            hydrolase with practical application value
  JOURNAL   Bioresour Technol 323, 124609 (2021)
   PUBMED   33387709
REFERENCE   2  (residues 1 to 359)
  AUTHORS   Kitadokoro,K., Kakara,M., Matsui,S., Osokoshi,R., Thumarat,U.,
            Kawai,F. and Kamitani,S.
  TITLE     Structural insights into the unique polylactate-degrading mechanism
            of Thermobifida alba cutinase
  JOURNAL   FEBS J 286 (11), 2087-2098 (2019)
   PUBMED   30761732
REFERENCE   3  (residues 1 to 359)
  AUTHORS   Ribitsch,D., Hromic,A., Zitzenbacher,S., Zartl,B., Gamerith,C.,
            Pellis,A., Jungbauer,A., Lyskowski,A., Steinkellner,G., Gruber,K.,
            Tscheliessnig,R., Herrero Acero,E. and Guebitz,G.M.
  TITLE     Small cause, large effect: Structural characterization of cutinases
            from Thermobifida cellulosilytica
  JOURNAL   Biotechnol Bioeng 114 (11), 2481-2488 (2017)
   PUBMED   28671263
REFERENCE   4  (residues 1 to 359)
  AUTHORS   Thumarat,U., Kawabata,T., Nakajima,M., Nakajima,H., Sugiyama,A.,
            Yazaki,K., Tada,T., Waku,T., Tanaka,N. and Kawai,F.
  TITLE     Comparison of genetic structures and biochemical properties of
            tandem cutinase-type polyesterases from Thermobifida alba AHK119
  JOURNAL   J Biosci Bioeng 120 (5), 491-497 (2015)
   PUBMED   25910960
REFERENCE   5  (residues 1 to 359)
  AUTHORS   Roth,C., Wei,R., Oeser,T., Then,J., Follner,C., Zimmermann,W. and
            Strater,N.
  TITLE     Structural and functional studies on a thermostable polyethylene
            terephthalate degrading hydrolase from Thermobifida fusca
  JOURNAL   Appl Microbiol Biotechnol 98 (18), 7815-7823 (2014)
   PUBMED   24728714
REFERENCE   6  (residues 1 to 359)
  AUTHORS   Thumarat,U., Nakamura,R., Kawabata,T., Suzuki,H. and Kawai,F.
  TITLE     Biochemical and genetic analysis of a cutinase-type polyesterase
            from a thermophilic Thermobifida alba AHK119
  JOURNAL   Appl Microbiol Biotechnol 95 (2), 419-430 (2012)
   PUBMED   22183084
REFERENCE   7  (residues 1 to 359)
  AUTHORS   Hu,X., Thumarat,U., Zhang,X., Tang,M. and Kawai,F.
  TITLE     Diversity of polyester-degrading bacteria in compost and molecular
            analysis of a thermoactive esterase from Thermobifida alba AHK119
  JOURNAL   Appl Microbiol Biotechnol 87 (2), 771-779 (2010)
   PUBMED   20393707
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF024151.6
            Evidence Source    :: EMBL-EBI
            Source Identifier  :: PF12740.13
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Pseudomonas aeruginosa"
                     /db_xref="taxon:287"
     Protein         1..359
                     /product="chlorophyllase/cutinase-like alpha/beta fold
                     protein"
                     /GO_function="GO:0050525 - cutinase activity [Evidence
                     IEA]"
                     /GO_function="GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /calculated_mol_wt=39256
     Region          62..>155
                     /region_name="alpha/beta hydrolases"
                     /note="A functionally diverse superfamily containing
                     proteases, lipases, peroxidases, esterases, epoxide
                     hydrolases and dehalogenases. The catalytic apparatus
                     typically involves three residues (catalytic triad): a
                     serine, a glutamate or aspartate and a...; cl21494"
                     /db_xref="CDD:473884"
ORIGIN      
        1 mrfrtlylvl cglllghaat aaepptaptp ptqpaegpgg adyrhasvhq whfgegarey
       61 wvftpeqpvp gnaplviflh gwsvmqpdly rawidhivrr gmvvvypryq pdlktpnaaf
      121 ldnaagalgd alrrlqagel gvrprlnqva ylghsaggll aanlaaaser lklpaakalm
      181 vvepgksqgk rwdgvaqerl sglakgtlll avcgdedshv actdakriyr qsrhippsdk
      241 nllllrsdrh gappllanha aptapvfgpq ypkeedsdwl ldrvekrlev qqaegrytgh
      301 dplvidaldw ygtwklfdgl tdaafynrnr qyalgatpeq tgmgqwsdgt pvkpikvlk