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MULTISPECIES: bifunctional transcriptional activator/DNA repair


LOCUS       WP_003113880             357 aa            linear   BCT 19-FEB-2025
            enzyme AdaA [Pseudomonas].
ACCESSION   WP_003113880
VERSION     WP_003113880.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 357)
  AUTHORS   Aravind,L., Anantharaman,V., Balaji,S., Babu,M.M. and Iyer,L.M.
  TITLE     The many faces of the helix-turn-helix domain: transcription
            regulation and beyond
  JOURNAL   FEMS Microbiol Rev 29 (2), 231-262 (2005)
   PUBMED   15808743
REFERENCE   2  (residues 1 to 357)
  AUTHORS   Rosinski,J.A. and Atchley,W.R.
  TITLE     Molecular evolution of helix-turn-helix proteins
  JOURNAL   J Mol Evol 49 (3), 301-309 (1999)
   PUBMED   10473770
REFERENCE   3  (residues 1 to 357)
  AUTHORS   Wintjens,R. and Rooman,M.
  TITLE     Structural classification of HTH DNA-binding domains and
            protein-DNA interaction modes
  JOURNAL   J Mol Biol 262 (2), 294-313 (1996)
   PUBMED   8831795
REFERENCE   4  (residues 1 to 357)
  AUTHORS   Morohoshi,F., Hayashi,K. and Munakata,N.
  TITLE     Molecular analysis of Bacillus subtilis ada mutants deficient in
            the adaptive response to simple alkylating agents
  JOURNAL   J Bacteriol 173 (24), 7834-7840 (1991)
   PUBMED   1744039
REFERENCE   5  (residues 1 to 357)
  AUTHORS   Morohoshi,F., Hayashi,K. and Munakata,N.
  TITLE     Bacillus subtilis ada operon encodes two DNA alkyltransferases
  JOURNAL   Nucleic Acids Res 18 (18), 5473-5480 (1990)
   PUBMED   2120677
REFERENCE   6  (residues 1 to 357)
  AUTHORS   Brennan,R.G. and Matthews,B.W.
  TITLE     The helix-turn-helix DNA binding motif
  JOURNAL   J Biol Chem 264 (4), 1903-1906 (1989)
   PUBMED   2644244
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11450612
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     Protein         1..357
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription
                     factor activity [Evidence IEA]"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /calculated_mol_wt=38992
     Region          9..356
                     /region_name="AdaA"
                     /note="Methylphosphotriester-DNA--protein-cysteine
                     methyltransferase (N-terminal fragment of Ada), contains
                     Zn-binding and two AraC-type DNA-binding domains
                     [Replication, recombination and repair]; COG2169"
                     /db_xref="CDD:441772"
ORIGIN      
        1 mshaalflpl pdddtlyaal lardpaydgf afvgvkstgv fcrltcpark pkrentlffd
       61 sikgcveagf rpclrcrple rlgtqgplvs dllqrlgrqp qrrwfeddla algydpstvr
      121 rafkrefgvt flemarlrrl gqvaerlssg arvidaqlda sfdsdsgfrs afarllgepp
      181 sqlrgrellk adwlqtplga mlavadaqal hlleffdrpa lsgelkrlqk ssgssigfgr
      241 fasidrieae ladyfggtpv rfqtplalna saftrtvwqa lrevpcgstq syaglarsig
      301 spssvravar anganqiaiv ipchrvigsd gsltgygggl wrkrwllehe rrmgaag