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LOCUS WP_003113773 431 aa linear BCT 23-DEC-2024 ACCESSION WP_003113773 VERSION WP_003113773.1 KEYWORDS RefSeq. SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (residues 1 to 431) AUTHORS Quistgaard,E.M., Low,C., Guettou,F. and Nordlund,P. TITLE Understanding transport by the major facilitator superfamily (MFS): structures pave the way JOURNAL Nat Rev Mol Cell Biol 17 (2), 123-132 (2016) PUBMED 26758938 REFERENCE 2 (residues 1 to 431) AUTHORS Zhang,X.C., Zhao,Y., Heng,J. and Jiang,D. TITLE Energy coupling mechanisms of MFS transporters JOURNAL Protein Sci 24 (10), 1560-1579 (2015) PUBMED 26234418 REFERENCE 3 (residues 1 to 431) AUTHORS Fukuda,M., Takeda,H., Kato,H.E., Doki,S., Ito,K., Maturana,A.D., Ishitani,R. and Nureki,O. TITLE Structural basis for dynamic mechanism of nitrate/nitrite antiport by NarK JOURNAL Nat Commun 6, 7097 (2015) PUBMED 25959928 REMARK Publication Status: Online-Only REFERENCE 4 (residues 1 to 431) AUTHORS Yan,N. TITLE Structural Biology of the Major Facilitator Superfamily Transporters JOURNAL Annu Rev Biophys 44, 257-283 (2015) PUBMED 26098515 REFERENCE 5 (residues 1 to 431) AUTHORS Yan,H., Huang,W., Yan,C., Gong,X., Jiang,S., Zhao,Y., Wang,J. and Shi,Y. TITLE Structure and mechanism of a nitrate transporter JOURNAL Cell Rep 3 (3), 716-723 (2013) PUBMED 23523348 REFERENCE 6 (residues 1 to 431) AUTHORS Yan,N. TITLE Structural advances for the major facilitator superfamily (MFS) transporters JOURNAL Trends Biochem Sci 38 (3), 151-159 (2013) PUBMED 23403214 REFERENCE 7 (residues 1 to 431) AUTHORS Law,C.J., Maloney,P.C. and Wang,D.N. TITLE Ins and outs of major facilitator superfamily antiporters JOURNAL Annu Rev Microbiol 62, 289-305 (2008) PUBMED 18537473 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 13023816 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..431 /organism="Pseudomonas aeruginosa" /db_xref="taxon:287" Protein 1..431 /product="nitrate/nitrite transporter" /GO_component="GO:0016020 - membrane [Evidence IEA]" /GO_function="GO:0022857 - transmembrane transporter activity [Evidence IEA]" /GO_process="GO:0055085 - transmembrane transport [Evidence IEA]" /GO_process="GO:0071705 - nitrogen compound transport [Evidence IEA]" /calculated_mol_wt=47123 Region 12..404 /region_name="MFS_NRT2_like" /note="Plant Nitrate transporter NRT2 family and Bacterial Nitrate/Nitrite transporters of the Major Facilitator Superfamily; cd17341" /db_xref="CDD:340899" Site order(22..23,26..27,30,62,112..113,115..117,120,138, 141..142,145,221,224..225,228..230,233,261,265,332..333, 337,341,356,359..360,363..364,367) /site_type="other" /note="putative chemical substrate binding pocket [chemical binding]" /db_xref="CDD:340899" ORIGIN 1 maslrqkqys vlgmstlsfa vnfavwtmfs iigirikdel glsesqfglm valpiltgsl 61 vrlplglitd rfggrivffi hmllvaipiy glafasqywh ylvlglfvgl aggsfavgia 121 ytsawfeker qgtamgifga gnagaaitnl vapmivvafg wrmvpqvysv amlvtavlfw 181 lftwtdpahl kgaaeasqrp nlakqlapla elrvwrfgly yffvfggfva lalwlpkyyi 241 aeygldlkta sfitmlftlp sgliralggw fsdhygarsv nwgvfwvclv clfflsypqt 301 tmtihgiqgd lslgiglnvw lftflvfvvg iaqgfgkasv yriihdyyps nmgtvggmvg 361 vigglggfcl pilfgyaadh igvrsscfml lfgltvvcmv wmhyaikqqr ridgqaasad 421 vdlpptlpth s