Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]
LOCUS WP_003113491 665 aa linear BCT 23-DEC-2024 ACCESSION WP_003113491 VERSION WP_003113491.1 KEYWORDS RefSeq. SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (residues 1 to 665) AUTHORS Akritidou,K. and Thurtle-Schmidt,B.H. TITLE OLD family nuclease function across diverse anti-phage defense systems JOURNAL Front Microbiol 14, 1268820 (2023) PUBMED 37840731 REMARK Publication Status: Online-Only REFERENCE 2 (residues 1 to 665) AUTHORS Schiltz,C.J., Adams,M.C. and Chappie,J.S. TITLE The full-length structure of Thermus scotoductus OLD defines the ATP hydrolysis properties and catalytic mechanism of Class 1 OLD family nucleases JOURNAL Nucleic Acids Res 48 (5), 2762-2776 (2020) PUBMED 32009148 REFERENCE 3 (residues 1 to 665) AUTHORS Schiltz,C.J., Lee,A., Partlow,E.A., Hosford,C.J. and Chappie,J.S. TITLE Structural characterization of Class 2 OLD family nucleases supports a two-metal catalysis mechanism for cleavage JOURNAL Nucleic Acids Res 47 (17), 9448-9463 (2019) PUBMED 31400118 REFERENCE 4 (residues 1 to 665) AUTHORS Snider,J., Thibault,G. and Houry,W.A. TITLE The AAA+ superfamily of functionally diverse proteins JOURNAL Genome Biol 9 (4), 216 (2008) PUBMED 18466635 REMARK Publication Status: Online-Only COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 11466458 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..665 /organism="Pseudomonas aeruginosa" /db_xref="taxon:287" Protein 1..665 /product="ATP-dependent nuclease" /EC_number="3.1.-.-" /GO_function="GO:0004519 - endonuclease activity [Evidence IEA]" /GO_function="GO:0004527 - exonuclease activity [Evidence IEA]" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_function="GO:0016887 - ATP hydrolysis activity [Evidence IEA]" /GO_function="GO:0046872 - metal ion binding [Evidence IEA]" /calculated_mol_wt=73651 Region <210..545 /region_name="YbjD" /note="Predicted ATP-dependent endonuclease of the OLD family, contains P-loop ATPase and TOPRIM domains [Replication, recombination and repair]; COG3593" /db_xref="CDD:442812" ORIGIN 1 mlalriflls ptrlnlrdvt ianwtkidll gseweagaep tvdldsllps ldvwldvplr 61 eiqhvvdilp tldwsggllg vrlafriknl dklkseyiaq rtaardaaiv gpngesikia 121 vwprnltdfl errlrahlel laypldpaav vppgrsglat pqrlpasala fehppfkkli 181 kideiaaqrd fadasssdgg edkgeannrr fkrrlsdqlr syydrhldpt ktpsdkdyea 241 lgaiqaaers fdarleegfs aafqeledlg ypgmsnpklk istllrgtdg lkhgssvqyq 301 vadpsgdgsn tlqlpedysg lgyqnliamv fmlmgfrdew mrvekagile gadvsheiqp 361 lhlvlveepe ahlhaqvqqv finkaytllr khadlgvaen yctqlivsth sshvaheadf 421 anlryfrrrp aasmgetptt tvanlsyifg dgdetkrfvk rylkathcdl ffadgvifve 481 gqaerilvph firhhfpels rryvtllelg gshvhsfrnl vdalgiatli igdldattav 541 kiidknmret trwksarpeq gkaqqtansv lkdwhpgkkl idelvalppd ghistaggdy 601 elyvayqkpv nvkgasedsd liiprtfeda lilenlavag kiegsitsak vrgivaqdls 661 gdegr