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MULTISPECIES: phosphonate ABC transporter substrate-binding protein


LOCUS       WP_003113119             334 aa            linear   BCT 20-NOV-2023
            [Pseudomonas].
ACCESSION   WP_003113119
VERSION     WP_003113119.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 334)
  AUTHORS   Rizk,S.S., Cuneo,M.J. and Hellinga,H.W.
  TITLE     Identification of cognate ligands for the Escherichia coli phnD
            protein product and engineering of a reagentless fluorescent
            biosensor for phosphonates
  JOURNAL   Protein Sci 15 (7), 1745-1751 (2006)
   PUBMED   16751609
REFERENCE   2  (residues 1 to 334)
  AUTHORS   Chen,C.M., Ye,Q.Z., Zhu,Z.M., Wanner,B.L. and Walsh,C.T.
  TITLE     Molecular biology of carbon-phosphorus bond cleavage. Cloning and
            sequencing of the phn (psiD) genes involved in alkylphosphonate
            uptake and C-P lyase activity in Escherichia coli B
  JOURNAL   J Biol Chem 265 (8), 4461-4471 (1990)
   PUBMED   2155230
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR03431.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     gene            1..334
                     /gene="phnD"
     Protein         1..334
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /EC_number="7.3.2.2"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_process="GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /calculated_mol_wt=36908
     Region          25..282
                     /region_name="PBP2_PnhD"
                     /note="Substrate binding domain of ABC-type phosphonate
                     uptake system; contains the type 2 periplasmic binding
                     fold; cd13575"
                     /db_xref="CDD:270293"
     Site            order(70,86,88,116,150..152,181,199,229)
                     /site_type="other"
                     /note="chemical substrate binding site [chemical binding]"
                     /db_xref="CDD:270293"
ORIGIN      
        1 mlkrfsrvla asallagsla gmahadqpvi nfgiistess qnlksiwepf lkdmsqqtgy
       61 qvkaffapdy agiiqgmrfd kvdiawygnk aameavdrah geifaqtvaa sgapgywsll
      121 iankdskids ledmlanaks ltfgngdpns tsgylvpgyy vfaknnvdpv kafkrtlnss
      181 hevnalavan kqvdvatfnt egmerleltq pekarqlkvi wksplipgdp lvwrnnlsde
      241 qknklrdfff kyganaeqkk vladlqwskf qpsdddqllp irqlelfkqr tdvannanlg
      301 aeekaaklka ldeelaklek rmaereqkta anag