MULTISPECIES: glutamate--cysteine ligase [Pseudomonas].

LOCUS       WP_003109990             527 aa            linear   BCT 25-NOV-2024
ACCESSION   WP_003109990
VERSION     WP_003109990.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 527)
  AUTHORS   Wongsaroj,L., Saninjuk,K., Romsang,A., Duang-Nkern,J.,
            Trinachartvanit,W., Vattanaviboon,P. and Mongkolsuk,S.
  TITLE     Pseudomonas aeruginosa glutathione biosynthesis genes play multiple
            roles in stress protection, bacterial virulence and biofilm
            formation
  JOURNAL   PLoS One 13 (10), e0205815 (2018)
   PUBMED   30325949
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 527)
  AUTHORS   Hibi,T., Nii,H., Nakatsu,T., Kimura,A., Kato,H., Hiratake,J. and
            Oda,J.
  TITLE     Crystal structure of gamma-glutamylcysteine synthetase: insights
            into the mechanism of catalysis by a key enzyme for glutathione
            homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 101 (42), 15052-15057 (2004)
   PUBMED   15477603
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR01434.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     gene            1..527
                     /gene="gshA"
     Protein         1..527
                     /product="glutamate--cysteine ligase"
                     /EC_number="6.3.2.2"
                     /GO_function="GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=59093
     Region          1..517
                     /region_name="GshA"
                     /note="Gamma-glutamylcysteine synthetase [Coenzyme
                     transport and metabolism]; COG2918"
                     /db_xref="CDD:442162"
ORIGIN      
        1 msdllsrrla llgaaanlpl lteclhgier eclrvdsdgk laltphpral gstlthpqit
       61 tdyseallef itptetdvad tlgdlerihr fasskldgey lwspsmpcel pdeesipiar
      121 ygssmigrlk yvyrkglalr ygktmqciag ihynfslper lwpllrqaeg selserdyqs
      181 aayialirnf rryswllmyl fgaspaldag flrgrpsqle rldehtlylp yatslrmsdl
      241 gyqnnaqagl tpcyndlqsy idslrqavst pyppyekvgt kqdgewvqln tnilqieney
      301 yssirpkrvt ytgerpvqal aargvqyvev rcldinpflp lgidldearf ldafllfcaf
      361 sdspllngec sdatdnflav vkegrrpglq lqrrgqpvel qvwaneller iadtaalldr
      421 arggeahaaa laaqrakvad aeltpsaqvl kvmrergesf eafslrqsre haeyfrqhpl
      481 aaeeqarfek masdslaeqt elerdqdgdf dtfvaayqas ilglisn