Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]
LOCUS WP_003103761 564 aa linear BCT 20-NOV-2023 ACCESSION WP_003103761 VERSION WP_003103761.1 KEYWORDS RefSeq. SOURCE Pseudomonas ORGANISM Pseudomonas Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae. REFERENCE 1 (residues 1 to 564) AUTHORS Liu,Y., Guo,X., Liu,W., Wang,J. and Kent Zhao,Z. TITLE Structural Insights into Malic Enzyme Variants Favoring an Unnatural Redox Cofactor JOURNAL Chembiochem 22 (10), 1765-1768 (2021) PUBMED 33523590 REFERENCE 2 (residues 1 to 564) AUTHORS Burley,K.H., Cuthbert,B.J., Basu,P., Newcombe,J., Irimpan,E.M., Quechol,R., Foik,I.P., Mobley,D.L., Beste,D.J.V. and Goulding,C.W. TITLE Structural and Molecular Dynamics of Mycobacterium tuberculosis Malic Enzyme, a Potential Anti-TB Drug Target JOURNAL ACS Infect Dis 7 (1), 174-188 (2021) PUBMED 33356117 REFERENCE 3 (residues 1 to 564) AUTHORS Bologna,F.P., Andreo,C.S. and Drincovich,M.F. TITLE Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure JOURNAL J Bacteriol 189 (16), 5937-5946 (2007) PUBMED 17557829 REFERENCE 4 (residues 1 to 564) AUTHORS Wang,J., Tan,H. and Zhao,Z.K. TITLE Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12 JOURNAL Protein Expr Purif 53 (1), 97-103 (2007) PUBMED 17215140 REFERENCE 5 (residues 1 to 564) AUTHORS Yang,Z., Zhang,H., Hung,H.C., Kuo,C.C., Tsai,L.C., Yuan,H.S., Chou,W.Y., Chang,G.G. and Tong,L. TITLE Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme JOURNAL Protein Sci 11 (2), 332-341 (2002) PUBMED 11790843 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 11486672 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..564 /organism="Pseudomonas" /db_xref="taxon:286" Protein 1..564 /product="NAD-dependent malic enzyme" /EC_number="1.1.1.38" /GO_function="GO:0004470 - malic enzyme activity [Evidence IEA]" /GO_function="GO:0004471 - malate dehydrogenase (decarboxylating) (NAD+) activity [Evidence IEA]" /GO_function="GO:0046872 - metal ion binding [Evidence IEA]" /GO_function="GO:0051287 - NAD binding [Evidence IEA]" /calculated_mol_wt=62289 Region 1..562 /region_name="PRK13529" /note="oxaloacetate-decarboxylating malate dehydrogenase" /db_xref="CDD:237414" ORIGIN 1 mtetakrply vphagpslle mpllnkgsaf stqeridfnl qgllphniet ieeqterays 61 qynlcntdld rhiflrsiqd nnetlffrll eehleemmpi iytptvgqac qefskiyrth 121 rglfisypdr eriddilrsa tknnvkivvv tdserilglg dqgiggmgip igklslytac 181 ggispaytlp vvldvgtnnp dllndpmymg wrhervsgaq yeefvdlfiq aikrrwpnvl 241 lqfedfaqtn ampllerykd elccfnddiq gtaavavgtl laackakgek lseqtvtfvg 301 agsagcgiae qiiaamqleg ldeaqarrri fmvdrwgllt ddmsnlldfq hrlaqkradl 361 gawggqqgdd lallevirna rptvligvsg qrglfseevi relhshckqp lvmplsnpts 421 rveatpqeil nwtdgqalva tgspfqpvqv gdkripiaqc nnayifpgig lgviaaranr 481 vtegmlmaaa nalancspiv tqgegavlpa lgdirevskr iavavakqaq aegkalhtsd 541 evlndaiean fwfpryrayr rtsf