MULTISPECIES: tRNA (N6-isopentenyl

LOCUS       WP_003093158             446 aa            linear   BCT 20-NOV-2023
            adenosine(37)-C2)-methylthiotransferase MiaB [Pseudomonas].
ACCESSION   WP_003093158
VERSION     WP_003093158.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 446)
  AUTHORS   Pierrel,F., Hernandez,H.L., Johnson,M.K., Fontecave,M. and Atta,M.
  TITLE     MiaB protein from Thermotoga maritima. Characterization of an
            extremely thermophilic tRNA-methylthiotransferase
  JOURNAL   J Biol Chem 278 (32), 29515-29524 (2003)
   PUBMED   12766153
REFERENCE   2  (residues 1 to 446)
  AUTHORS   Pierrel,F., Bjork,G.R., Fontecave,M. and Atta,M.
  TITLE     Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein
  JOURNAL   J Biol Chem 277 (16), 13367-13370 (2002)
   PUBMED   11882645
REFERENCE   3  (residues 1 to 446)
  AUTHORS   Anantharaman,V., Koonin,E.V. and Aravind,L.
  TITLE     TRAM, a predicted RNA-binding domain, common to tRNA uracil
            methylation and adenine thiolation enzymes
  JOURNAL   FEMS Microbiol Lett 197 (2), 215-221 (2001)
   PUBMED   11313137
REFERENCE   4  (residues 1 to 446)
  AUTHORS   Esberg,B., Leung,H.C., Tsui,H.C., Bjork,G.R. and Winkler,M.E.
  TITLE     Identification of the miaB gene, involved in methylthiolation of
            isopentenylated A37 derivatives in the tRNA of Salmonella
            typhimurium and Escherichia coli
  JOURNAL   J Bacteriol 181 (23), 7256-7265 (1999)
   PUBMED   10572129
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR01574.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     gene            1..446
                     /gene="miaB"
     Protein         1..446
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /EC_number="2.8.4.3"
                     /GO_function="GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster
                     binding [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence
                     IEA]"
                     /calculated_mol_wt=49847
     Region          2..442
                     /region_name="MiaB"
                     /note="tRNA A37 methylthiotransferase MiaB [Translation,
                     ribosomal structure and biogenesis]; COG0621"
                     /db_xref="CDD:440386"
ORIGIN      
        1 makklfieth gcqmneydss rmadllgehq alevtenaae advillntcs irekaqekvf
       61 sklgmwrelk qqnpdlvigv ggcvasqega airerapyvd vvfgpqtlhr lpemidaars
      121 trkpqvdvsf peiekfdrlp eprvdgptaf vsvmegcsky csfcvvpytr geevsrpfdd
      181 viaevihlae ngvrevtllg qnvngfrglt hdgrladfae llrvvaavdg ieriryttsh
      241 plefsdaliq ahaevpelvk fihlpvqsgs drvlaamkrn htvleyksri rklkaavpdi
      301 cissdfivgf pgetekdfeq tmklvedvgf dfsfsfiysa rpgtpaadla ddlpeevkkq
      361 rlqilqsrih qqgyeisrrm vgstqrilvt dfskkdpgml qgrtennriv nfrcdnprli
      421 gqfaqvhidd alphslrgtl idstlh