MULTISPECIES: IscS subfamily cysteine desulfurase [Pseudomonas].

LOCUS       WP_003092832             404 aa            linear   BCT 01-JUL-2024
ACCESSION   WP_003092832
VERSION     WP_003092832.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 404)
  AUTHORS   Ikeuchi,Y., Shigi,N., Kato,J., Nishimura,A. and Suzuki,T.
  TITLE     Mechanistic insights into sulfur relay by multiple sulfur mediators
            involved in thiouridine biosynthesis at tRNA wobble positions
  JOURNAL   Mol Cell 21 (1), 97-108 (2006)
   PUBMED   16387657
REFERENCE   2  (residues 1 to 404)
  AUTHORS   Lauhon,C.T., Skovran,E., Urbina,H.D., Downs,D.M. and Vickery,L.E.
  TITLE     Substitutions in an active site loop of Escherichia coli IscS
            result in specific defects in Fe-S cluster and thionucleoside
            biosynthesis in vivo
  JOURNAL   J Biol Chem 279 (19), 19551-19558 (2004)
   PUBMED   14978044
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR02006.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     Protein         1..404
                     /product="IscS subfamily cysteine desulfurase"
                     /EC_number="2.8.1.7"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA]"
                     /GO_process="GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /calculated_mol_wt=44536
     Region          1..404
                     /region_name="PRK14012"
                     /note="IscS subfamily cysteine desulfurase"
                     /db_xref="CDD:184450"
     Site            order(75..76,79,152,180,183,203,206)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:99746"
     Site            206
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99746"
ORIGIN      
        1 mklpiyldys attpvdprva qkmsecllmd gnfgnpasrs hvfgwkaeea venarrqvae
       61 lvnadpreiv wtsgatesdn laikgvahfy sgkgkhiits kiehkavldt crqleregfe
      121 vtylepgedg litpalveaa lrddtilvsv mhvnneigtv ndiaaigelt rsrgvlfhvd
      181 aaqstgkvei dldklkvdlm sfsahktygp kgigalyvrr kprvriegqm hggghergmr
      241 sgtlathqiv gmgeafriak eemaqenarv lalrdrffaq idgleelyin gsmtsrvphn
      301 lnvsfnyveg eslimalkdl avssgsacts aslepsyvlr algrndelah ssirftfgrf
      361 tteeeidyaa kkvveavskl relsplwdmy kegvdlsqve wqah