MULTISPECIES: class-II fumarase/aspartase family protein

LOCUS       WP_003092055             483 aa            linear   BCT 18-FEB-2025
            [Pseudomonas].
ACCESSION   WP_003092055
VERSION     WP_003092055.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 483)
  AUTHORS   Yang,J., Wang,Y., Woolridge,E.M., Arora,V., Petsko,G.A.,
            Kozarich,J.W. and Ringe,D.
  TITLE     Crystal structure of 3-carboxy-cis,cis-muconate lactonizing enzyme
            from Pseudomonas putida, a fumarase class II type cycloisomerase:
            enzyme evolution in parallel pathways
  JOURNAL   Biochemistry 43 (32), 10424-10434 (2004)
   PUBMED   15301541
REFERENCE   2  (residues 1 to 483)
  AUTHORS   Fujii,T., Sakai,H., Kawata,Y. and Hata,Y.
  TITLE     Crystal structure of thermostable aspartase from Bacillus sp.
            YM55-1: structure-based exploration of functional sites in the
            aspartase family
  JOURNAL   J Mol Biol 328 (3), 635-654 (2003)
   PUBMED   12706722
REFERENCE   3  (residues 1 to 483)
  AUTHORS   Williams,S.E., Woolridge,E.M., Ransom,S.C., Landro,J.A.,
            Babbitt,P.C. and Kozarich,J.W.
  TITLE     3-Carboxy-cis,cis-muconate lactonizing enzyme from Pseudomonas
            putida is homologous to the class II fumarase family: a new
            reaction in the evolution of a mechanistic motif
  JOURNAL   Biochemistry 31 (40), 9768-9776 (1992)
   PUBMED   1390752
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 10107773
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     Protein         1..483
                     /product="class-II fumarase/aspartase family protein"
                     /calculated_mol_wt=52797
     Region          42..478
                     /region_name="pCLME"
                     /note="prokaryotic 3-carboxy-cis,cis-muconate
                     cycloisomerase (CMLE)_like; cd01597"
                     /db_xref="CDD:176469"
     Site            order(56..58,61..62,105,108..109,132..133,185..187,
                     189..190,192..193,199,201,206,209,212..213,216,230..232,
                     235,240..241,256..257,262..263,265..266,268..269,272,
                     275..276,283,286..287,292..293,295,317..318,323,330..331,
                     336,340,345..349,355..356,358,360,399,466..467,471,474)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:176469"
     Site            order(113..114,139..141,186..187,229,315..316,318,349)
                     /site_type="active"
                     /db_xref="CDD:176469"
ORIGIN      
        1 mdaeslemla rssctahpqs fggapaessc khehshivds kthgggyagp vsrrifcshc
       61 rlqrwldvea alalaqadvg vippeaalei akaarlsavd lqqiadgvas tghslmpllg
      121 alqrncspsa reyvhfgatt qdiqdtaqsl emrdvldete raldilldrl svlasgtrna
      181 lmvarthsip alpttfglkv agwidellrh rerlgearkr ilvvqlfggv gtmaafgdea
      241 mlllesfarr lsldvplagw hvsrdrvtef vstlamvtas lariadeirt lnrweigele
      301 vgwtqqqigs stmphkrnpe gceqvvvlar lakaqvllal damileherd yrgtrlewca
      361 vadvshyalm alslltdtid gltvhdetma rtanaysdai cteafvfema kqlgkssaye
      421 iifeitqacq rkripmrqal esdprvgavm pketlarlfe prthlgmagt ivdkvldkva
      481 shh