MULTISPECIES: PaaI family thioesterase [Pseudomonas].

LOCUS       WP_003086091             135 aa            linear   BCT 31-DEC-2024
ACCESSION   WP_003086091
VERSION     WP_003086091.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 135)
  AUTHORS   Song,F., Zhuang,Z., Finci,L., Dunaway-Mariano,D., Kniewel,R.,
            Buglino,J.A., Solorzano,V., Wu,J. and Lima,C.D.
  TITLE     Structure, function, and mechanism of the phenylacetate pathway hot
            dog-fold thioesterase PaaI
  JOURNAL   J Biol Chem 281 (16), 11028-11038 (2006)
   PUBMED   16464851
REFERENCE   2  (residues 1 to 135)
  AUTHORS   Dillon,S.C. and Bateman,A.
  TITLE     The Hotdog fold: wrapping up a superfamily of thioesterases and
            dehydratases
  JOURNAL   BMC Bioinformatics 5, 109 (2004)
   PUBMED   15307895
  REMARK    Publication Status: Online-Only
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 10130874
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     Protein         1..135
                     /product="PaaI family thioesterase"
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /GO_function="GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /calculated_mol_wt=14491
     Region          20..133
                     /region_name="PaaI_thioesterase"
                     /note="PaaI_thioesterase is a tetrameric acyl-CoA
                     thioesterase with a hot dog fold and one of several
                     proteins responsible for phenylacetic acid (PA)
                     degradation in bacteria. Although orthologs of PaaI exist
                     in archaea and eukaryotes, their function has not...;
                     cd03443"
                     /db_xref="CDD:239527"
     Site            order(49,78,85..88)
                     /site_type="other"
                     /note="CoenzymeA binding site [chemical binding]"
                     /db_xref="CDD:239527"
     Site            order(50,52,55,79..83,85)
                     /site_type="other"
                     /note="subunit interaction site [polypeptide binding]"
                     /db_xref="CDD:239527"
     Site            order(51,59..60,63..64,78)
                     /site_type="active"
                     /note="PHB binding site [active]"
                     /db_xref="CDD:239527"
ORIGIN      
        1 mnaaqvqali rmglpmaedi dfrverlder halaripfhg klvrpggtls gptimalada
       61 amyavilgrl gavemavtsn lninflskpr pedllaeasi lklgrrqvvc evgvfsqsne
      121 edlvahvtgt yalpl