MULTISPECIES: dihydrofolate reductase [Pseudomonas].

LOCUS       WP_003084430             168 aa            linear   BCT 01-JAN-2025
ACCESSION   WP_003084430
VERSION     WP_003084430.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 168)
  AUTHORS   Francis,K. and Kohen,A.
  TITLE     Protein motions and the activation of the CH bond catalyzed by
            dihydrofolate reductase
  JOURNAL   Curr Opin Chem Biol 21, 19-24 (2014)
   PUBMED   24742825
REFERENCE   2  (residues 1 to 168)
  AUTHORS   Schnell,J.R., Dyson,H.J. and Wright,P.E.
  TITLE     Structure, dynamics, and catalytic function of dihydrofolate
            reductase
  JOURNAL   Annu Rev Biophys Biomol Struct 33, 119-140 (2004)
   PUBMED   15139807
REFERENCE   3  (residues 1 to 168)
  AUTHORS   Gready,J.E.
  TITLE     Dihydrofolate reductase: binding of substrates and inhibitors and
            catalytic mechanism
  JOURNAL   Adv Pharmacol Chemother 17, 37-102 (1980)
   PUBMED   7004143
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11087044
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     Protein         1..168
                     /product="dihydrofolate reductase"
                     /EC_number="1.5.1.3"
                     /GO_function="GO:0004146 - dihydrofolate reductase
                     activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /calculated_mol_wt=18072
     Region          5..167
                     /region_name="DHFR_1"
                     /note="Dihydrofolate reductase; pfam00186"
                     /db_xref="CDD:425512"
     Site            order(8,25,30,60,104,110,123)
                     /site_type="other"
                     /note="folate binding site [chemical binding]"
                     /db_xref="CDD:238127"
     Site            order(10,17,47..49,67,105..108)
                     /site_type="other"
                     /note="NADP+ binding site [chemical binding]"
                     /db_xref="CDD:238127"
ORIGIN      
        1 marplamiaa lgenraigid nrlpwrlpad lkhfkamtlg kpvimgrktw dslgrplpgr
       61 lnlvvsrqag lalegaevfa sldaalarae awaqaedade lmliggaqly aealpraarl
      121 yltrvglape gdaffpeidg aawrlassie haaaddapay afevwerr