MULTISPECIES: 5,6-dimethylbenzimidazole synthase [Pseudomonas].

LOCUS       WP_003082592             218 aa            linear   BCT 20-NOV-2023
ACCESSION   WP_003082592
VERSION     WP_003082592.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 218)
  AUTHORS   Taga,M.E., Larsen,N.A., Howard-Jones,A.R., Walsh,C.T. and
            Walker,G.C.
  TITLE     BluB cannibalizes flavin to form the lower ligand of vitamin B12
  JOURNAL   Nature 446 (7134), 449-453 (2007)
   PUBMED   17377583
REFERENCE   2  (residues 1 to 218)
  AUTHORS   Gray,M.J. and Escalante-Semerena,J.C.
  TITLE     Single-enzyme conversion of FMNH2 to 5,6-dimethylbenzimidazole, the
            lower ligand of B12
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 104 (8), 2921-2926 (2007)
   PUBMED   17301238
REFERENCE   3  (residues 1 to 218)
  AUTHORS   Rodionov,D.A., Vitreschak,A.G., Mironov,A.A. and Gelfand,M.S.
  TITLE     Comparative genomics of the vitamin B12 metabolism and regulation
            in prokaryotes
  JOURNAL   J. Biol. Chem. 278 (42), 41148-41159 (2003)
   PUBMED   12869542
REFERENCE   4  (residues 1 to 218)
  AUTHORS   Pollich,M. and Klug,G.
  TITLE     Identification and sequence analysis of genes involved in late
            steps in cobalamin (vitamin B12) synthesis in Rhodobacter
            capsulatus
  JOURNAL   J. Bacteriol. 177 (15), 4481-4487 (1995)
   PUBMED   7635831
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR02476.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     gene            1..218
                     /gene="bluB"
     Protein         1..218
                     /product="5,6-dimethylbenzimidazole synthase"
                     /EC_number="1.13.11.79"
                     /GO_function="GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /calculated_mol_wt=23870
     Region          8..210
                     /region_name="Nitro_FMN_reductase"
                     /note="nitroreductase family protein; cl00514"
                     /db_xref="CDD:444950"
     Site            order(21,23,41,43..44,46,49..50,52,54,56,58..63,134,
                     136..137,140..141,144..145,157..158,168)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380311"
     Site            order(23,25,27,53,157..158)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:380311"
ORIGIN      
        1 msdsshafsa teraavyrai aerrdmrhfs ggsvapella rlleaahqap svglmqpwrf
       61 iritepalrr aihglveeer grtaealger sdefmrlkve gvldcaellv aalmddrerh
      121 vfgrrtlpqm dlaslacaiq nlwlasraeg lgmgwvslfd paalgellgl ppgseavavl
      181 clgpvaefyp apmlalegws eprplaelvy qnrwgera